毛黄堇药材的质量标准研究

Study on Quality Standard of Corydalis tomentella

目的:建立毛黄堇药材的质量标准。方法:从原植物形态、性状特征、显微特征、薄层色谱(TLC)方面对药材进行定性鉴别;检测药材水分、总灰分和水浸出物的含量;采用高效液相色谱法测定药材中脱氢卡维丁的含量:色谱柱为Agilent C_(18),流动相为乙腈-磷酸盐缓冲液(含20 mmol/L磷酸二氢钾、10 mmol/L二乙胺、0.1%磷酸)(28∶72,V/V),流速为1.0 mL/min,检测波长为347nm,柱温为35℃,进样量为10μL。结果:原植物为多年生草本,药材常皱缩成团,被毛,主根圆锥形,根茎和根部有明显的干裂,叶卷曲而多碎落,花呈黄白色;花粉粒类圆形,可见方晶和柱晶,非腺毛众多,可见具缘纹孔、螺纹及网纹导管,亦可见木纤维。药材样品TLC图斑点清晰,分离度好。水分为7.5%~18.5%,总灰分为20.5%~26.2%,水浸出物为29.9%~46.4%。脱氢卡维丁检测进样量线性范围为0.040 08~2.404 8μg(r=0.999 9),精密度、稳定性、重复性试验的RSD<2.0%;加样回收率为95.6%~102.5%(RSD=2.3%,n=9)。结论:该研究所建标准可用于毛黄堇药材质量标准评价。

OBJECTIVE： To establish the quality standard of Corydalis tomentella. METHODS： The medicinal material was identified in respects of property, microscopic characteristics and TLC. The contents of moisture, total ash and water soluble ex- tract were determined. The content of dehydrocavidine was determined by HPLC. The determination was performed on Agilent C18 column with mobile phase consisted of acetonitrile-pbosphate buffer solution （containing 20 mmol/L monopotassium phosphate, 10 mmol/L diethylamine, 0.1% phosphoric acid）（28： 72, V/V） at the flow rate of 1.0 mL/min. The detection wavelength was set at 347 nm, and the column temperature was 35 %. The sample size was 10μL. RESULTS： The original plant is perennial herbs. The medicinal material often shrank into a ball. The main root was in conical shape; the rhizomes and roots were distinctly chapped; leaves curled and broken, and flowers were yellowish-white. The pollen grains were round-like in shape; square and columnar crys- tals were found; there were a large number of nonglandular hairs. The bordered pit, threaded and reticulate catheter were found; wood fiber was also found. TLC spots were clear and well-separated. The content of moisture were 7.5%-18.5%, total ash were 20.5%-26.2% ,and extract were 29.9%-46.4%. The linear range of dehydrocavidine were 0.040 08-2.404 8 μg （r=0.999 9） ; RSDs of precision, stability and reproducibility tests were lower than 2.0%. The recoveries were 95.6%-102.5% （RSD=2.3%, n=9）. CONCLUSIONS ： The established standard can be used for quality evaluation of C tomentella.