电化学指纹图谱法快速鉴别三七

Rapid Identification of Notoginseng by Nonlinear Chemical Fingerprinting

目的:利用中药作为电化学反应底物,建立三七的鉴别方法。方法:采用H^+-Mn^(2+)-CH^3COCH^3-BrO^3为振荡体系,以一定量的中药粉末为反应底物,反应温度控制为310 K,系统搅拌速度为600 r·min^(-1)。以饱和甘汞电极作为参比电极,铂电极为指示电极,采用电化学工作站记录化学振荡体系中电位E随时间t的变化,利用Origin8.0软件绘制E-t曲线,获得电化学指纹图谱。结果:电化学指纹图谱的重复性良好,三七及其伪品的电化学指纹图谱具有明显的差别。结论:可以用已建立的电化学指纹图谱法鉴别三七。

Objective： To establish the method for identification of notoginseng by nonlinear chemical fingerprinting. Using traditional Chinese herbal medicine as substrate of electrochemical reaction. Method： In a glass reactor with appropriate amount of Chinese herbal medicine powder as the substrate of reaction, the H^＋-Mn^2＋-CH3COCH^3-BrO3 for oscillation system. Temperature kept at 310 K and the stirring speed was 600 r·min^-1. With saturated calomel electrode as reference, the platinum electrode as indicator electrode, the electrochemical workstation records of chemical potential in the oscillation system, along with the change of time. Draw E-t curve using origin 8.0 software, get the non-linear electrochemical fingerprint. Result： The repeatability is good, the non-linear electrochemical fingerprint intuitively see the party to participate in the non-linear electrochemical fingerprint has obvious difference, also have different characteristic parameters, sample cluster analysis divided into two categories. Conclusion： This method can be used to identify notoginseng.