雷公藤中雷公藤红素分离纯化工艺研究

Study on Isolation and Purification Technologies of Celastrol from Tripterygium wilfordii

目的:探索了雷公藤中活性成分雷公藤红素的分离纯化工艺。方法:将经过粉碎处理雷公藤植物根皮,以氯仿为提取剂,超声提取2 h,所得浸膏经过石油醚脱脂,再用醇提水沉法,得到雷公藤红素粗品。粗品先经过硅胶柱分离,再用重结晶方法进行纯化。结果:并考察了不同的溶剂体系重结晶的分离效果。采用石油醚/乙酸乙酯比例为9.2︰0.8重结晶溶剂的效果最佳,所得产物的纯度达到98.2%。结论:本文的雷公藤红素分离纯化方法,工艺简单,产品的质量和纯度较好,具有一定的参考应用价值。

Objective： Exploring the isolation and purification technologies of active components celastrol from Tripterygium wi/lbrdii. Methods： The extraction was followed degreasing with petroleum ether and alcohol extracting-water precipitating process after crushing the roots of Triptervgium wilfordii were extracted under ultrasonic condition for 2 h using CHCl3 as extractant. The pure celastrol was obtained employing silica gel column and recrystallizing process. Results： The effect of different solvent systems was investigated in the paper. While using the ratio of petroleum ether / ethyl acetate to 9.2 ： 0.8 as recrystallization solvent was the best, and the purity of the product was over 98.2 %. Conclusion： The isolation and purification technologies were simple and easy to treat, gave product with good quality and purity and showed certain reference value.