摘要
花生四烯酸(Arachidonic acid,ARA或AA)是一种重要的多不饱和脂肪酸,有多种生理功能和保健功效。近年来,利用高山被孢霉(Mortierella alpina)发酵生产ARA已成为研究热点,其具有产率高、安全性好、生产易控制等特点,深受国内外研究者青睐。如何提高ARA产量及分离纯化ARA是利用M.alpina发酵生产ARA的关键技术。该文论述了M.alpina发酵过程中提高ARA产量的主要方式,即发酵条件优化、菌体老化和代谢调控,以及常用的从M.alpina中分离纯化ARA的方法,并对其研究前景进行了展望。
Arachidonic acid （ARA or AA）, one of the most important polyunsaturated fatty acids （PUFAs）, has various physiological activities and positive effects on human health. ARA production by Mortierella alpina has become a hot topic in recent years, owing to that it is effective, safe and easy to control. How to improve ARA yield and purification efficiency is important to ARA production in M. alpina. Therefore, in this review, we summarized some methods to improve ARA yield： optimization of culture conditions, mycelium aging technologies and metabolic regulation, and the commonly used methods for ARA isolation and purification, to provide a theoretical basis for ARA production by M. alpina fermentation.
基金
Supported by Natural Science Foundation of Yunnan Province(2016FB030)
Young Academic and Technical Talent Program of Yunnan Province(2011CI027)~~
关键词
高山被孢霉
花生四烯酸
发酵
代谢调控
分离纯化
Mortierella alpina
Arachidonic acid
Fermentation
Metabolic regulation
Punhcation
