基因KLF4在人源Her2高表达转基因小鼠中的表达研究（英文）

Study on Expression of KLF4 in Her2 Over-expressed Transgenic Mice

[目的]研究人源Her2高表达转基因小鼠的生物学特性,并检测基因KLF4的表达变化。[方法]在以往构建的Her2高表达转基因小鼠稳定遗传体系基础上,传代扩大种群。用PCR方法鉴定子代基因型,绘制体重-周龄曲线。RT-PCR方法检测基因KLF4(FL)及其分型KLF4α的表达。[结果]Her2高表达乳腺癌阳性小鼠体内基因在乳腺癌发生过程中总表达量略微下降;基因的表达量与正常小鼠相比明显增加;然而在乳腺癌阳性小鼠体内KLF4总基因的表达量却呈下降趋势。[结论]该研究能填补KLF4在动物模型水平上研究的空白,使KLF4与乳腺癌发生发展关系的研究更具完整性。

[Objective]The aim was to study the biological characteristics of Her2 over-expressed transgenic mice and to detect the changes in expression of KLF4. [Method] On the basis of establishment of Her2 over-expressed transgenic mice, the population was expanded by propagation. The genotype of the offspring was identified by PCR, the body weight-age curve was drawn, and the expression of KLF4 （FL） and KLF4α was detected by RT-PCR. [Result] In the Her2-overex-pressed breast cancer-positive mice, the expression level of KLF4α decreased slightly in the course of breast cancer, and the expression level of KLF4 （FL） was increased significantly compared with those in normal mice. However, the expression level of KLF4 gene in the breast cancer-positive mice showed a downward trend. [Conclusion] This study will fill the gaps in the study of KLF4 at the animal model level and complete the study on relationship between KLF4 and the occurrence and development of breast cancer.