甘草黄酮的提取分离及其葡萄糖苷酶抑制活性研究

Extraction and Separation of Licoflavone and Its Inhibitory Activity on Glucosaccharase

采用超声提取和碱溶酸沉法提取甘草的活性成分甘草黄酮;通过化学显色法进行定性鉴定,采用分光光度法测定甘草黄酮含量;并建立了一种基于电化学法的葡萄糖苷酶抑制活性筛选新方法,以临床降糖药阿卡波糖验证了电化学筛选法的可靠性。结果表明,甘草黄酮具有较好的葡萄糖苷酶抑制活性,其IC50为67.6mg·L^(-1)。葡萄糖苷酶抑制活性电化学筛选法具有良好的准确性和抗干扰能力,为中药葡萄糖苷酶抑制剂的研发提供了有价值的参考。

We extracted active constituent licoflavone from Radix liquiritiae using ultrasonic extraction and alkali-soluble acid precipitation extraction methods. We qualitatively identified it by a chemical coloration meth- od,and determined its content by spectrophotometry. Moreover, we established a new glucosaccharase inhibitor screening method based on an electrochemical amperometric method. And we demonstrated the reliability of electrochemical screening method by clinical hypoglycemic drug acarbose. The results show that the licoflavone has good glucosaccharase inhibitory activity and its ICs0 is 67.6 mg ~ L 1. The electrochemical screening method for a-glucosidase inhibition activity has high accuracy and anti-interference. This study provides a valuable refer- ence for screening glucosaccharase inhibitor from Chinese tradition medicine.