Galectin-3促进甲状腺乳头状癌细胞转移机制的研究

The role of Galectin-3 in promoting metastasis of papillary thyroid carcinoma

背景与目的:甲状腺乳头状癌(papillary thyroid cancer,PTC)是人体常见的肿瘤之一。半乳凝集素-3(Galectin-3)是一种参与调控肿瘤发生、发展的多功能蛋白。Galectin-3广泛表达于多种肿瘤组织及肿瘤患者的血清中。该研究旨在探索Galectin-3调控PTC细胞转移的相关机制。方法:对40例PTC组织切片进行Galectin-3染色及评分。对PTC细胞B-cpap行无血清培养后分别检测细胞内外的Galectin-3的表达水平。用两种不同方法抑制Galectin-3在细胞内外的表达后利用CCK8法及细胞划痕法检测细胞功能的改变。利用免疫荧光及共聚焦摄像技术观察在细胞内敲低Galectin-3表达后细胞形态的改变。结果:颈部淋巴结转移患者组织切片的Galectin-3染色评分显著高于临床无转移者(P<0.05)。抑制细胞内外Galectin-3的表达均能抑制PTC细胞迁移,但对于增殖的影响不同。敲低细胞内Galectin-3表达后,PTC细胞形态发生改变。结论:PTC患者组织中的Galectin-3表达与肿瘤转移相关。抑制细胞内外的Galectin-3表达均能显著降低肿瘤细胞的迁移。

Background and purpose： Papillary thyroid cancer （PTC） is one of the most common carcinomas in humans. Galectin-3 is a multifunctional protein which regulates different process of tumorigenesis. Galectin-3 is widely distributed in various cancer tissues and the sera of cancer patients. The aim of this study was to explore the role of Galectin-3 in promoting PTC cells migration. Methods： Immunohistochemical analyses were performed on 40 PTC tissues and scores were calculated. PTC B-cpap cells were harvested after serum-free culture, and the extracellular and intracellular levels of Galectin-3 were measured. CCK-8 assay and wound healing assay were conducted after inhibition of Galectin-3 with two different methods. The transformation of cell morphology after inhibition of intracellular Galectin-3 was analyzed using immunofluorescence and confocal laser scanning. Results： Compared with those PTC patients without cervical lymph node metastasis, immunohistochemical scores of Galectin-3 were significantly higher in those with cervical lymph node metastases （P〈0.05）. The inhibition of extracellular and intracellular Galectin-3 attenuated the migration ability of cancer cells, but had different impact on cell proliferation. Transformation of cell morphology was also observed after inhibition of intracellular Galectin-3. Conclusion： Galectin-3 expression in tumor tissues was related to PTC metastasis. Inhibition of extracellular and intracellular galectin-3 could significantly decrease PTC cell migration.