青蒿琥酯对猴免疫缺陷病毒感染中国恒河猴模型T淋巴细胞活化的调节作用

Regulating Effects of Artesunate on SIV-infected Chinese Rhesus Macaque T Lymphocyte Activation

目的:观察注射用青蒿琥酯对猴免疫缺陷病毒(simian immunodeficiency virus,SIV)感染中国恒河猴模型免疫异常激活的改善情况,探讨青蒿琥酯对艾滋病的干预作用。方法:选用8只SIV感染平台期中国恒河猴模型,随机分为治疗组和对照组,每组各4只,治疗组按每天3 mg·kg^(-1)剂量肌肉注射青蒿琥酯注射液8周,对照组肌肉注射等剂量生理盐水。分别于给药前,给药后2周、4周、6周、8周,停药后4周、8周检测血浆病毒载量、全血细胞计数、淋巴细胞亚群及免疫活化相关标记物CD_(69)^+、CD_(25)^+、HLA-DR^+和CD_(38)^+表达情况。结果:青蒿琥酯可在4周内下调CD_8^+细胞CD_(69)^+、HLA-DR^+和CD_(38)^+表达水平(P<0.05),对CD_4^+细胞影响较小。给药第4周出现一过性嗜中性粒细胞下降,后回升至正常水平。结论:青蒿琥酯可改善SIV感染中国恒河猴细胞毒性T细胞免疫异常激活,嗜中性粒细胞下降是可逆的。

Objective： To observed the effect of artesunate on improvement of immunological abnormalities in Chinese rhesus monkeys infected with SIV to explore the use of artesunate on AIDS. Methods： Eight SIV-infected Chinese rhesus macaque in plateau phase were included and seperated equally in 2 groups. Treatment group were injected with artesunate on a dose of 3 mg·kg^（-1）·day for 8 weeks,while the control group were injected with normal saline. Tests were took at week 0,2,4,6,8,12 and 16,including blood routine,plasma viral load,T cell subsets,CD（69）^＋,CD（25）^＋,HLA-DR and CD（38）^＋. Results： Artesunate down regulated the exprssion level of CD（69）^＋,HLA-DR and CD（38）^＋ on CD8^＋ T cells within 4 weeks. Neutrophilic granulocyte went through a temporary drop at week4 and then returned to a normal level. Conclusion： Artesunate relieves the abnormal activation of cytotoxic T cells in SIV-infected Chinese rhesus macaques. Neutropenia in artesunate treatment is reversible.