养心2号方对慢性心力衰竭模型大鼠血清炎性细胞因子的影响

Effect of Yangxin No.2 Prescription on Serum Inflammatory Cytokines of Chronic Heart Failure Model Rats

目的:观察养心2号方对心力衰竭模型大鼠血清促炎因子:肿瘤坏死因子-α(tumor necrosis factor-alpha,TNF-α)、白细胞介素-1β(interleukin 1β,IL-1β)、白细胞介素-6(interleukin,IL-6)和IL-10的影响,探讨其治疗慢性心力衰竭(chronic cardiac failure,CHF)的作用机制。方法:将SD大鼠随机分为正常对照组、心力衰竭模型组,将采用皮下注射盐酸异丙肾上腺素法制备成功的慢性心力衰竭大鼠模型随机分为3组:模型组、卡维地洛组、养心2号方组。给予正常对照组、模型组大鼠等容蒸馏水灌胃,卡维地洛组予卡维地洛片0.562 5 mg·(kg·d)^(-1),灌胃;养心2号方组给予养心2号方中药汤剂灌胃。各组均连续给药4周,实验结束后观察各组实验大鼠心脏彩超及细胞因子含量的变化。结果:(1)药物干预后卡维地洛组、养心2号方组大鼠的心脏左室收缩末期内径、左室舒张末期内径均小于模型组(P<0.05);左室射血分数、左室短轴缩短率均大于模型组(P<0.05)。(2)药物干预后卡维地洛组、养心2号方组大鼠的血清促炎性因子与模型组比较,差异有统计学意义(P<0.05);与卡维地洛组比较,养心2号方组的IL-1β浓度显著降低(P<0.05);与模型组比较,卡维地洛组与养心2号方组IL-10均显著升高(P<0.01)。结论:养心2号方能够明显改善心力衰竭大鼠的心功能,抑制大鼠的促炎性细胞因子异常激活,提高抑炎性因子的水平,调节免疫功能,从而延缓心室重塑。

Objective： To observe the effect of Yangxin No. 2 Prescription on serum levels of pro-inflammatory cytokines（ TNF-α,IL^-1,IL-6） and serum anti-inflammatory factors（ IL-10） in treatment of Chronic heart failure rats with heart failure and to explore the mechanism of it. Methods： Methods： SD rats were randomly divided into normal control group and heart failure model group. The chronic heart failure model rats which were prepared by subcutaneous injection of isoproterenol hydrochloride were randomly divided into 3 groups： model group,carvedilol Group,Yang Xin No. 2 prescription group. The rats in the normal model group and the rats in the model group were fed with distilled water; the rats in the carvedilol Group was given 0. 562 5 mg·（ kg·d）^-1 in treatment; the Yang Xin No. 2 prescription group was treated with traditional Chinese medicine decoction. The rats in each group were treated for 4 weeks. After the experiment,the changes of cardiac ultrasonography and cytokine contents were observed. Results：（1）The left ventricular systolic diameter and left ventricular end diastolic diameter in treatment group were significantly lower than that in model group（ P〈0. 05）,while the left ventricular ejection fraction and the shortening rate of short axis was higher than that of the model group（ P〈0. 05）. Compared with that the carvedilol group,the serum pro-inflammatory cytokines in the carvedilol group and the Yangxin No. 2 Prescription group were significantly different from those in the model group respectively（ P〈0. 05）. Compared with that of the carvedilol group,the serum pro-inflammatory cytokines in the carvedilol group and the Yangxin No. 2 Prescription group were significantly different from those in the model group（ P〈0. 05）. Compared with that of the carvedilol group,the concentration of IL-1β in the Yangxin No. 2 Prescription group was significantly lower（ P〈0. 05）. Compared with that of the model group,the levels of IL-10 in the carvedilol group and the Yangxin No. 2 Prescription group were significantly higher（ P〈0. 01）. Conclusion： Yangxin No. 2 Prescription can obviously improve the cardiac function of rats with Chronic heart failure,inhibit the abnormal activation of pro-inflammatory cytokines in rats,improve the level of anti-inflammatory factors,regulate immune function,and delay the ventricular remodeling.