脂肪酸去饱和酶1基因多态性、深海鱼油及其交互作用与口腔鳞状细胞癌的关系

Fatty acid desaturase 1 polymorphism,marine fish oil intake and the risk of oral squamous cell carcinoma: A case-control study

目的探讨脂肪酸去饱和酶1(FADS1)基因多态性、深海鱼油摄入及其交互作用与口腔鳞状细胞癌(OSCC)的关系。方法 2010年9月—2014年9月,采用病例-对照研究设计,收集福建医科大学附属第一医院经病理确诊的口腔鳞状细胞癌新发病例259例和按年龄、性别频数匹配的对照538例,采用自制调查表收集研究对象的一般情况、饮食习惯如深海鱼油摄入情况等资料,利用Taq Man SNP探针检测FADS1 rs174549基因型。应用非条件Logistic回归模型评估FADS1 rs174549基因多态性与深海鱼油摄入对口腔鳞状细胞癌发病风险的影响,并进行基因-环境交互作用分析。结果携带FADS1 rs174549 AA基因型可显著降低口腔鳞状细胞癌的发生风险(共显性模型:OR=0.53,95%CI 0.33~0.85;隐性模型:OR=0.57,95%CI 0.38~0.87)。与未补充深海鱼油者相比,规律地补充深海鱼油可以降低OSCC的发生风险(OR=0.54,95%CI 0.32~0.91)。此外,FADS1 rs174549基因多态性与补充深海鱼油之间存在正相乘交互作用(OR_(相乘)=0.31,95%CI 0.11~0.87)。结论FADS1rs174549 AA基因型和深海鱼油摄入是口腔鳞状细胞癌发生的保护因素,且二者之间存在相乘交互作用。

Objective To evaluate the independent and joint effects of FADS1 polymorphism and fish oil intake on oral squamous cell carcinoma （OSCC）. Methods A case-control study was conducted with 259 newly diagnosed primary OSCC patients and 538 controls frequency-matched by sex and age in Fujian from September 2010 to September 2014. Data on demographics and dietary habits such as marine fish oil intake were collected using a structure questionnaire. FADS1 rs174549 genotype was determined using TaqMan genotyping assays. Unconditional logistic regression was used to the odds ratios （ORs） and their 95% confidence intervals （CI） of FADS1 polymorphism and fish oil intake for OSCC. Results FADS1 rs174549 AA genotype was associated with decreased risk of OSCC （ codominant model ： OR = 0.53, 95% CI O. 33 - 0. 85 ; recessive model：OR = 0.57, 95% CI O. 38 -0.87）. Compared with those who non-intake marine fish oil, regularly intake of marine fish oil decreased the risk of OSCC（ OR = 0.54, 95% CI： 0. 32 -0.91 ）. Moreover, a muhiplicative interaction between FADS1 rs174549 polymorphism and marine fish oil intake for oral cancer （ OR mu,,iOioa,w = 0. 31, 95% CI 0. 11 -0.87）. Conclusion FADS1 rs174549 polymorphism and marine fish oil intake may be independent protective factors for OSCC with a gene-diet multiplicative interaction.