长链非编码RNA、环境因素与高血压的关系

Relationships among long noncoding RNA,environmental factors and hypertension

目的了解高血压主要环境影响因素,并探讨高血压与外周血白细胞长链非编码RNA(long noncoding RNA,lncRNA)的关系。方法采用病例-对照研究设计,利用分层随机抽样的方法收集福州市某医院2014年11月—2015年6月确诊的高血压患者和对照人群各382例,进行问卷调查、健康体检和实验室检测。采用实时荧光定量PCR方法检测长链非编码RNA(NR_104181和NR_027032)在高血压组和对照组中的表达情况。采用多因素Logistic回归模型分析环境因素、长链非编码RNA与高血压患病的关联。结果高血压环境影响因素的Logistic回归分析结果显示:饮食偏咸(OR=1.732,95%CI 1.204~2.493)、焦虑(OR=1.746,95%CI 1.326~2.298)、体质指数(BMI)≥24(OR=1.475,95%CI 1.030~2.111)、腹型肥胖(OR=1.836,95%CI 1.229~2.742)及高血压家族史(OR=3.004,95%CI 2.118~4.261)是高血压患病的危险因素,工作体力活动(OR=0.776,95%CI 0.620~0.972)是其保护因素。秩和检验结果提示:高血压组外周血白细胞中NR_104181表达水平高于对照组(Z=-2.144,P=0.032);高血压组外周血白细胞中NR_027032表达水平低于对照组(Z=-3.467,P=0.001)。高血压环境因素和遗传因素的Logistic回归分析结果显示:在控制饮食偏咸、焦虑、腹型肥胖和工作体力活动后,外周血白细胞中NR_104181高表达(OR=2.658,95%CI 1.109~6.370)和NR_027032低表达(OR=0.179,95%CI 0.057~0.560)可能与高血压患病存在关联。结论高血压是环境因素和遗传因素共同作用的结果,长链非编码RNA可能与高血压患病存在关联。

Objective To understand the main environmental factors of hypertension, and to explore the relationship between hypertension and leukocyte long noncoding RNA.Methods A case-control study was conducted in a hospital in Fuzhou City by using stratified random sampling method between Nov. 2014 and Jun. 2015 including 382 essential hypertension patients and 382 control subjects. The related surveys included questionnaire investigation, physical examination and laboratory detection. Real-time PCR method was used to compare the expression of long non-coding RNA （NR_104181 and NR_027032） in the case group with that of in the control group. Multiple factor Logistic regression was used to analyze the association of environmental factors, long noncoding RNA and the risk of hypertension. Results Multiple factor Logistic regression analysis revealed that the main risk factors of hypertension included： salty diet （ OR = 1. 732, 95% CI1.204-2.493）, anxiety （ OR =1. 746 , 95% CI 1.326 -2.298）, BMI 〉I 24 （OR = 1. 475, 95% CI 1. 030 -2. 111） , abdominal obesity（ OR = 1. 836, 95% CI 1. 229 -2. 742） , family history of hypertension （ OR = 3. 004, 95% CI 2. 118 - 4. 261 ）. The main protective factor of hypertension included ： physical activity （ OR = 0. 776, 95% CI 0. 620-0. 972）. Wilcoxon rank sum test indicated that： The expression level of NR_ 104181 in hypertension was higher than that in control group, the difference was statistically significant （P 〈 0.05 ）. The expression level of NR_027032 in hypertension was lower than that in control health group, the difference was statistically significant （P 〈 0.05）. Multiple factor Logistic regression analysis showed that controlling for salty diet, anxiety, abdominal obesity and physical activity, peripheral blood leukocytes NR_ 104181 high expression （ OR = 2. 658, 95% CI 1. 109 - 6. 370） , NR_027032 low expression （OR =0. 179, 95% CI O. 057 -0. 560） were possibly related to the risk of hypertension. Conclusion Hypertension is the result of environmental factors and genetic factors, and lneRNA may be associated with the risk of hypertension.