摘要
目的:分析神经营养素(NTs)家族成员及其酪氨酸激酶受体(Trk)在卵巢癌中的表达情况及其与患者总生存期(OS)的关系,评价其预后价值。方法:利用KaplanMeier plotter(KM plotter)在线数据库收集卵巢癌患者病理信息,分析NTs家族成员(NGF、BDNF、NT-3、NT-5)及其受体Trk(TrkA、TrkB、TrkC)表达与卵巢癌患者预后及临床病理参数的相关性。收集新鲜卵巢癌组织和癌旁组织各6例,Western blot法检测NTs及Trk蛋白表达。结果:BDNF mRNA高表达与卵巢癌患者较好的总生存期(OS)相关(危险比HR=0.78,95%CI为0.68~0.89,P<0.001);NGF、NT-3、NT-5及受体TrkA、TrkB、TrkC表达与卵巢癌患者的总生存期(OS)无关(P>0.05)。BDNF mRNA表达与临床分期Ⅰ、Ⅱ及Ⅲ期、病理分级Ⅱ级、组织学分型浆液性癌及TP53野生型的卵巢癌患者的OS密切相关(P<0.05)。NGF、NT-3及TrkA表达均与卵巢癌患者的临床病理参数不相关(P>0.05)。NT-5表达与病理分级Ⅱ级的卵巢癌患者的OS相关(P<0.05)。TrkB及TrkC表达与临床分期Ⅲ期的卵巢癌患者的OS密切相关(P<0.05)。Western blot法检测显示,NTs家族中NGF、BDNF、NT-5蛋白在卵巢癌组织中过表达,NT-3蛋白则为低表达;Trk家族中TrkA、TrkB及TrkC在卵巢癌组织中高表达。结论:BDNF表达与卵巢癌患者的预后密切相关,高表达患者的预后更好,可作为潜在的预后指标进行研究;NTs家族其他成员及受体Trk家族均无此预测作用。
Objective :To investigate the expressions of Neurotrophins (NTs) and tyro- sine kinase receptor (Trk) in ovarian cancer and its correlation with overall survival ( OS), and to evaluate its prognosis value. Methods:Kaplan-Meier plotter (KM plotter) online database was used to collect patients with ovarian cancer, and to analyze the correlation among the ex- pressions of NTs ( NGF, BDNF, NT-3, NT-5 ) and its receptors Trk (TrkA,TrkB, TrkC) and the prognosis and clinical pathological parameters of patients. 6 cases of fresh ovarian cancer tissues and adjacent tissues were collected and NTs and Trk proteins expression were detected by West- ern blot. Results:The mRNA high expression level of BDNF was associated with bette" overall survival (OS) for ovarian cancer patients (P〈0. 001 ) , hazard ratio (HR) was 0.78,95 % CI was 0.68 - 0.89. The expressions of NGF, NT-3, NT-5 and receptors TrkA, TrkB and TrkC were not correlated with the overall survival of patients with ovarian cancer (P〉0.05). The ex- pression of BDNF mRNA was closely related to the OS for patients with clinical stage Ⅰ , Ⅱ and Ⅲ,grade Ⅱ ,serous and TP53 wild type (P〈0.05). The expressions of NGF,NT-3 and Tr- kA were not correlated with the clinicopathological parameters of ovarian cancer (P〉0.05). However, the expression of NT-5 was correlated to the OS for patients with grade Ⅱ (P〈0.05) , and the expressions of TrkB and TrkC were closely related to the OS for patients with stage Ⅲ (P〈0.05). Western blot results showed that NGF, BDNF and NT-5 were over-expressed in o- varian cancer tissues, and NT-3 was down-regulated. TrkA, TrkB and TrkC were all over-ex- pressed in ovarian cancer tissues. Conclusion:The expression of BDNF is closely related to the prognosis of ovarian cancer patients, and high expression of BDNF has better prognosis. BDNF will be used as a potential prognostic indicator for cancer. But other members of NTs family and its receptors Trk family have no such predictive effect.
作者
曾小芳
赵俊暕
宋丽丽
王雅红
董友玲
肖艳平
Zeng Xiaofang;Zhao Junjian;Song Lili;et al(The Second People's Hospital of Fengrun District, Tangshan 063030;The Affiliated Hospital of North China University of Science and Technology, Tangshan 063000;Tangshan Health Center for Women and Children, Tangshan 063000)
出处
《现代妇产科进展》
CSCD
北大核心
2017年第11期810-815,共6页
Progress in Obstetrics and Gynecology