芩红乳房灌注剂的镇痛抗炎作用及急性毒性研究

Analgesic and anti-niflammatory effects and toxicity of Qinhong breast perfusion agent

采用"扭体法"和"热板法"研究了"芩红乳房灌注剂"的镇痛作用,用二甲苯致小鼠耳廓肿胀试验研究了该药的抗炎作用,并通过急性毒性试验对其安全性进行评价,测出了该药的最大给药量与最大耐受倍数。镇痛试验结果表明,"芩红乳房灌注剂"高剂量组、中剂量组、低剂量组和安痛定组的扭体反应次数分别为28.8±2.3,29.6±3.1,38.8±2.3和13.9±2.6,均显著低于生理盐水组(P<0.01或P<0.05);"热板法"试验在60 min和90 min时高剂量、中剂量与低剂量组、对照组相比差异显著(P<0.05),且在给药120 min低剂量的芩红乳房灌注剂和生理盐水组差异不显著(P>0.05),而高、中剂量与生理盐水组相比差异显著(0.01<P<0.05),说明低剂量组的镇痛作用持续时间较高剂量、中剂量短。抗炎试验结果显示3个药物组均有一定的肿胀抑制作用,高、中剂量组和阿司匹林组的肿胀度分别为(0.070±0.014)mm,(0.084±0.010)mm和(0.062±0.012)mm,均极显著低于生理盐水组(P<0.01),低剂量组的肿胀度与生理盐水组差异不显著(P>0.05),但是仍表现出11.11%的肿胀抑制率。急性毒性试验未测出药物的半数致死量(LD_(50)),最大给药量为80 g/kg,最大耐受倍数为临床奶牛拟用剂量的2 000倍。结果显示,芩红乳房灌注剂具有良好的镇痛、抗炎作用且应用安全。

The analgesic effect of＂Qinhong breast perfusion agent＂was investigated by writhing method and hot plate method.The auricle swelling test of xylene in mice was used to study the anti-inflammatory effect of the medicine.The acute toxicity test was performed,and the maximum dose of the drug was measured.Analgesic test results showed that the numbers of writhing of high dose group,middle dose group,low dose group and Antondin group were 28.8±2.3,29.6±3.1,38.8±2.3 and 13.9±2.6,respectively,significantly lower than the saline group（P〈0.01 or P〈0.05）.In the hot plate assay,there was a significant difference between the low-dose group and the high-dose group at 60 min and 90 min（P〈0.05）,while there was no significant difference between thelow dose of the perfusion of the red kidney and the normal saline group at 120 min（P〉0.05）,while there was a significant difference between the high dose and the middle dose groups（0.01P〈0.05）.It indicated that the analgesic effect of the low-dose group had a shorter duration time than the high dose and the middle dose groups.The anti-inflammatory test showed that the three groups had certain swelling inhibition,and the swelling degrees of high dose group,middle dose group and aspirin group were（0.070 ± 0.014） mm,（0.084 ± 0.010） mm and（0.062 ± 0.012） mm,significantly lower than the saline group（P〈0.01）.However,the swelling degree of the low dose group was not significantly different from that of the saline group（P〉0.05）,but the swelling inhibition rate was still 11.11%.The acute toxicity test failed to measure the LD50 of the drug.The maximum dose was 80 g/kg,and the maximal tolerance was 2000 times that of using on clinical cows.The results showed that the＂Qinhong breast perfusion agent＂has good analgesic and anti-inflammatory effects,and is safe enough to use.