响应曲面法优化超声辅助提取虎杖中白藜芦醇的工艺研究

Optimization of ultrasonic-assisted extraction technology of resveratrol from Polygonum Cuspidatum by response surface methodology

目的优化虎杖中白藜芦醇的提取工艺。方法以虎杖为原料,运用超声辅助提取技术对虎杖中的白藜芦醇进行提取,以乙醇溶液为溶剂,考察溶剂浓度、时间、温度、功率、料液比等因素对提取效果的影响,在单因素实验的基础上,通过响应曲面法优化提取工艺。结果最佳工艺参数为:乙醇浓度62.85%,超声时间37.92 min,温度60℃,料液比1:19.45(g/mL),超声功率240 W,此条件下白藜芦醇的提取得率为4.65%。结论本研究改进白藜芦醇的提取工艺,缩短提取时间,提高提取得率,可为白藜芦醇的提取和开发应用提供参考。

Objective To optimize the extraction process of resveratrol from Polygonum Cuspidatum. Methods The extraction of resveratrol from Polygonum Cuspidatum was achieved by ultrasound assistance. The effects of solvent concentration, ultrasound treatment time, extraction temperature, ultrasound power and material/liquid ratio on extraction efficiency were investigated using ethanol solution as solvent. On the basis of single-factor experiments, the extraction process was optimized by response surface methodology. Results The optimum conditions for extracting resveratrol from Polygonum Cuspidatum were as follows： ethanol concentration was 62.85%, ultrasonic time was 37.92 min, extraction temperature was 60 ,℃ material/liquid ratio was 1：19.45 （g/mL）, and ultrasound power was 240 W. Under these optimum conditions, the yield of resveratrol was 4.65%. Conclusion This study improves the extraction process of resveratrol, shortens the extraction time and improves the extraction yield, which can provide references for the extraction and development of resveratrol.