膜芯片用于肉制品中动物源性成分检测的研究

Application of film chip for detection of animal-derived components in meat products

目的研究膜芯片技术检测肉类及其制品中动物源性成分,验证该技术的准确性及可行性。方法利用膜芯片检测肉制品中的动物源性成分,对样品中猪、牦牛、驴及羊源性成分的灵敏度、检出限进行相关实验,并就实际样品的检测与国家标准进行比较。结果该技术用于动物源性成分检测特异性良好,4种动物源性检测的灵敏度为0.1 ng,检出限为0.1%(w:w),适用的样品种类较广,实际样品的检测结果与国家标准荧光PCR法一致。结论该技术可作为快速筛选的方法,为肉制品的动物源性成分鉴定和掺假检测提供理论依据。

Objective To investigate the application of film chip technology for detection of animal-derived components in meat and its products, and to verify the accuracy and feasibility of the technology. Methods Animal-derived components in meat products were detected by using film chip, the sensitivity and limit of detection （LOD） for components of Sus scrofa domestica, Bos mutus, Equus asinus and Ovis aries were tested in samples, and the detection results of actual samples by film chip were compared with those by the national standards. Results The technique had good specificity for detection of animal-derived components. The sensitivities of the technology was 0.1 ng and the LODs were 0.1% （w：w） for the 4 kinds of animal-derived components. The technology was applicable to a wide range of samples. The test results of the actual samples by film chip were in accordance with those by national standard of fluorescence PCR method. Conclusion This technique can be used as a rapid screening method, which provides theoretical basis for adulteration detection and identification of animal-derived components in meat products.