贞术消积汤抑制STAT3/Survivin信号通路诱导肝癌细胞自噬实验研究

Expenimental Study of Zhenzhu Xiaoji Decoction Inducing Autophagy via STAT3/Survivin Signal Pathway in Hepatoma Cells

目的:通过观察贞术消积汤作用H22移植瘤后细胞形态学变化,探讨该复方体内抗肿瘤的分子机制。方法:将小鼠肝癌细胞移植于ICR小鼠皮下,贞术消积汤灌胃给药10 d;采用苏木素-伊红(HE)染色,光镜下观察各组细胞形态的变化;应用透射电镜观察模型组和贞术消积汤组细胞超微结构的改变;荧光定量PCR法检测Survivin mRNA表达;免疫组化法检测信号转导与转录活化因子3(STAT3)蛋白和抗凋亡因子(Survivin)蛋白表达。结果:光镜下观察发现,用药组细胞排列稀疏,组织产生大量空泡;透射电镜观察可见,贞术消积汤组细胞内发现自噬体和自噬溶酶体;与模型对照组相比,贞术消积汤组Survivin mRNA低于模型组;贞术消积汤组STAT3和Survivin表达下调。结论:贞术消积汤体内抗肿瘤的作用机制可能与其诱导细胞自噬有关,并通过抑制STAT信号通路发挥作用。

Objective ： To explore the in vivo antitumor mechanism of Zhenzhu Xiaoji decoction by detecting the morphology changes of H22 cells. Methods： Murine hepatocarcinoma cells were injected into ICR mice and then they were given Zhenzhu Xiaoji decoction once a day for ten continuous days. The changes of cell mor- phology were observed under light microscopy and transmission electron microscopy（TEM） ; the expression of Survivin mRNA was detected by RT - PCR; and the protein expressions of STAT3 and Survivin were observed by IHC. Results： Under light microscopy, cell lineage was sparse and lots of vacuoles were produced in tis- sues with the medication; under TEM, there were autophagosome and autolysosome in cells; the expression of Survivin, Survivin mRNA, and STAT3 were down- regulated in the medication group compared to the model control group. Conclusion： The in vivo antitumor effect of Zhenzhu Xiaoji decoction is probably related to in- ducing cell autophagy by inhibiting STAT3/Survivin signal pathway.