意大利蜜蜂AmMRJP5基因的克隆及原核表达

Cloning of AmMRJP5 Gene from Apis mellfera L. and Its Prokaryotic Expression

从意大利蜜蜂Apismellfera L.的cDNA中克隆出MRJP5基因(简称AmMRJP5),构建pPAL7-AmMRJP5表达载体,并将其转入大肠杆菌原核表达系统,通过抗生素筛选和IPTG诱导获得重组蛋白的高效表达菌株,最后通过SDS-PAGE电泳和Western blot免疫印迹鉴定,确定AmMRJP5蛋白已在大肠杆菌中成功表达。

In this paper,the coding region of AmMRJP5 was amplified by PCR from Apis mellifera L. cDNA and cloned into the prokaryotic expression vector pPAL7, which for fusion expression in E. coli. Through antibiotics screening and IPTG inducing, the efficient expression of recombinant proteins strain was obtained. And the SDS-PAGE and Western blot results showed that the AmMRJP5 was successfully expressed in E. coli.