楹树提取物对大鼠醋酸型慢性胃溃疡的治疗作用及抗炎机制

Therapeutic effects of extract of Albizia chinensis (Osbeck) Merr. on rats with acetic acid-induced chronic gastric ulcer and anti-inflammatory mechanism

目的研究楹树提取物对大鼠醋酸型慢性胃溃疡的治疗作用及其抗炎机制。方法将50只雄性Wistar大鼠随机分为5组:正常对照组、模型组、法莫替丁组、楹树提取物100和200 mg/kg组。用30%醋酸诱导大鼠慢性胃溃疡,连续给药12 d,观察大鼠一般行为状态和胃组织形态并测定胃溃疡面积,综合评价药物抗胃溃疡作用。将48只雄性Wistar大鼠随机分为3组:正常对照组、模型组、楹树提取物200 mg/kg组。手术后连续给药3 d后处死大鼠,取溃疡部位的胃组织,观察胃组织病理学变化,ELISA测定胃组织中炎性因子TNF-α和IL-6的浓度,邻联茴香胺法测定髓过氧化物酶(MPO)活性,Western印迹法检测胃组织P38和JNK的磷酸化水平。结果与模型组相比,楹树提取物200 mg/kg组大鼠溃疡面积减少38.4%(P<0.05),胃组织中TNF-α和IL-6的浓度分别降低43.8%(P<0.01)和32.7%(P<0.01),MPO活性降低37.3%(P<0.01),P38磷酸化水平下调(P<0.01),而JNK磷酸化水平、P38及JNK总蛋白水平均无明显改变。结论楹树提取物(200 mg/kg)显著改善醋酸损伤引起的胃组织结构破坏,对大鼠醋酸型慢性胃溃疡具有一定的治疗作用,其作用机制可能是通过抑制p38炎症信号通路的过度激活从而减轻胃组织炎症反应,JNK通路未参与该过程。

Objective To investigate the anti-ulcer effects of Albizia chinensis（Osbeck）Merr. extract on rats with acetic acid-induced chronic gastric ulcer and explore the anti-inflammatory mechanism. Methods Wistar male rats（n=50）were randomized in-to five groups：control,model,famotidine,Albizia chinensis extract 100 and 200 mg/kg groups. In the experiment,30%acetic acid was used to induce chronic gastric ulcer in rats. The anti-ulcer effects of A. chinensis extract were evaluated by measuring the changes in general behavior of rats,gastric tissue morphology and the ulcer area after 12 d treatments. Wistar male rats（n=48）were random-ized into three groups：control,model and A. chinensis extract（200 mg/kg）groups. At 3 d after operation,the animals were sacrificed and stomachs were rapidly removed and processed for subsequent assays. The contents of TNF-αand IL-6 were evaluated by ELISA. The activity of myeloperoxidase（MPO）was tested by o-dianisidine method. Furthermore Western blot was used to examine the phos-phorylation of P38 and JNK. Results Compared with the model group,the gastric ulcer areas of rats were reduced by 38.4%（P〈0.05）and the activity of MPO declined by 37.3%（P〈0.01）in A. chinensis extract（200 mg/kg）group. Meanwhile the contents of TNF-αand IL-6 in A. chinensis extract（200 mg/kg）group respectively decreased by 43.8%（P〈0.01）and 32.7%（P〈0.01）com-pared with the model group. A. chinensis extract（200 mg/kg）obviously suppresed the phosphorylation of P38（P〈0.01）,but phos-phorylation of JNK and expression of P38 and JNK remained nearly unaltered. Conclusion A. chinensis extract（200 mg/kg）exhibits a protective effect on acetic acid-induced chronic gastric ulcer,which might be relevant to anti-inflammation by inhibiting the p38 sig-naling pathway but not JNK signaling pathway. A. chinensis extract（200 mg/kg）has no effect on JNK signaling pathway.