正交实验法优选忍冬藤中绿原酸和马钱苷的提取工艺研究

Optimizing the Extraction Process of Chlorogenic Acid and Loganin in Caulis Lonicerae by Orthogonal Test Method

目的:研究忍冬藤中绿原酸和马钱苷的提取工艺。方法:以绿原酸和马钱苷浓度(质量分数)为评价指标,对提取方法、溶剂浓度、药材粒度进行单因素考察,并采用L_9(3~4)正交实验设计对提取工艺进行优选。结果:综合考虑,最佳提取工艺为回流提取法:回流次数为2次,乙醇浓度为60%,回流时间为2h,液料比15:1。结论:正交实验优选工艺条件稳定可行,快速简便,可为工业化大生产提供一定的理论依据。

Objective：To study the extraction process of chlorogenic acid and loganin in Caulis Lonicerae.Methods： Using the total concentration of chlorogenic acid and loganin as the reference,the single factor（i.e.,extraction methods, concentrations of extraction solvent and the material granularity）experiment and Lg（34）orthogonal test were carried out to optimize the extraction conditions.Results：The optimized extraction conditions are as follows：reflux extraction method, 60% ethanol,liquid/solid ratio 15：1,reflux 2 times with 2 h for each.Conclusion：The optimized extraction conditions are fast, stable and feasible,and provide theoretical basis for industrial production.