子痫前期相关miR-18a对人正常滋养细胞浸润功能影响的研究

The study of affect of preeclampsia related mi R-18a on the invasion of human trophoblast cell

目的通过研究子痫前期相关微小RNA-18a(micro RNA-18a,mi R-18a)对人正常滋养细胞(HTR8)浸润功能的影响,探讨mi R-18a与子痫前期疾病的相关性及其在发病过程中的作用机制。方法将mi R-18a前体分子分组转染HTR8细胞,并分为三组:pre-mi RNA阴性对照组(NC)、mi R-18a过表达组和mi R-18a抑制组;反转录PCR检测转染后各组中mi R-18a m RNA的表达水平;侵袭小室实验(Transwell)检测转染后各组细胞浸润能力的变化情况。结果与NC组(0.407±0.019)比较,mi R-18a过表达组抑制效果显著(0.884±0.061;0.161±0.014),差异具有统计学意义(P<0.05);与NC组(66.67±3.05)比较,抑制mi R-18a的表达可明显降低细胞的浸润能力(29.631±3.112,P<0.05)。结论 mi R-18a能够在一定程度上影响人正常滋养细胞的浸润功能,可能与子痫前期的发病相关。

Objective To investigate the effect of preeclampsia（PE） related miR-18a on the invasion capacity of human normal trophoblast cell line （HTR-8）, and study the further relationship between PE with miR-18a and the possible pathogenesis mechanism. Methods The synthesized miR-18a was transfected into HTR-8 ceils. And dbided into three experimental parts： miR-18a over-expression group, miR-18a inhibition group and pre-miRNA un-transfection group （NC）. The expression of miR-18a in these three groups were detected by reverse transcription PCR （RT-PCR）. The proliferation and apoptosis capacity of HTR-8 cell of each groups were checked by Transwell assay. Results There were significant differences on the expressions of miR-18a mRNA among over-expression group （0.88 ± 0.06）, inhibition group （0.88 ±0.06） and NC group （0.407 ±0.019） （P 〈 0.05）. Compared with NC group, invasion of HTR-8 cell was significantly changed in inhibition group, with significant difference （29.61 ±3.11 ,P 〈 0.05）. Conclusions The miR-18a could effect the invasion capacity of trophoblast cell, and have certain relationship with the pathogenesis of PE.