辣木组织培养技术初步研究

Preliminary Study of Tissue Culture Technology of Moringa oleifera Lam.

[目的]初步研究辣木组织培养技术。[方法]以辣木种子为外植体,通过组织培养诱导不定芽,形成完整的组培再生植株,并研究辣木诱导、增殖和生根的适宜培养基和培养条件。[结果]最佳外植体诱导培养基为1/2MS+6-BA0.80 mg/L+NAA0.40 mg/L+益培灵0.10 g/L,萌芽率为95%;最佳增殖培养基为MS+6-BA0.30 mg/L+NAA0.02 mg/L+益培灵0.05 g/L,增殖个数为6.4个;最佳生根培养基为1/2MS+IBA0.30 mg/L+ABT1号0.015 mg/L+益培灵0.03 g/L,生根率可达95%,根长为5.21 cm。[结论]辣木组培快繁技术有助于解决良种缺乏的问题,并为种质资源开发利用提供技术支撑。

[Objective]To preliminarily study the tissue culture technology of Moringa oleifera Lam..[Method]Seeds of M.oleifera Lam.were used as explants to induce adventitious buds and form a complete tissue culture plant by tissue culture technology.The suitable medium and cul-ture condition for induction, multiplication and rooting of M.oleifera Lam.were studied.[Result]The most suitable culture combination for ex-plants induction was 1/2MS＋6-BA0.80 mg/L＋NAA0.40 mg/L＋Yipeiling 0.10 g/L,the germination rate was 95%.The best culture combi-nation for multiplication was MS＋6-BA0.30 mg/L＋NAA0.02 mg/L＋Yipeiling 0.05 g/L,the number of multiplication was 6.4.The best rooting medium was 1/2MS＋IBA0.30 mg/L＋ABT10.015 mg/L＋Yipeiling 0.03 g/L,the rooting rate could reach 95%and the root length was 5.21 cm.[Conclusion]Rapid propagation technology of M.oleifera Lam.can help to solve the lack of seeds and provide technical support for de-velopment and utilization of germplasm resources.