两种细菌降解内蒙古褐煤过程及液相产物分析

Degradation Process and Liquid Products Analysis of Inner Mongolia Lignite by Two Kinds of Bacteria

以少动鞘脂单胞菌和红城红球菌对氧化的内蒙古褐煤(氧化煤)进行了降解实验研究.通过测定这两种细菌每天降解氧化煤后离心液相产物的pH值和降解率,发现降解率与pH值有密切的联系,当pH值在8.1~8.2之间,两种细菌均表现出较好的降解效果;当pH值大于8.4时,细菌的生物活性降低,导致氧化煤被降解效率受到抑制.少动鞘脂单胞菌和红城红球菌的最大降解率分别为50.86%和52.86%,说明这两种细菌均能有效降解氧化煤,其中红城红球菌的降解率高于少动鞘脂单胞菌的降解率.通过紫外光谱(UV)、红外光谱(IR)和气相色谱-质谱联用(GCMS)等方法对降解的液相产物进行分析,结果表明,两种细菌对氧化煤降解的液相产物主要包含酯类和酰胺类物质.

Oxidized Inner Mongolia lignite was degraded by two kinds of bacteria Sphingomonas Polyaromaticivorans and Rhodococcus Erythropolis.The pH value and degradation rate of centrifugal liquid product of degradated oxidized lignite were detected every day.The results show that the degradation rate is closely related to the pH value,when the pH value is in the range of 8.1 and 8.2,both bacteria show better degradation effect,and the degradation rate is inhibited when the pH value is greater than 8.4.The degradation rates of Sphingomonas Polyaromaticivorans and Rhodococcus Erythropolis is 50.86%and 52.86%,respectively,which indicate that both of these bacteria can effectively degrade oxidized lignite,and the degradation rate of Rhodococcus Erythropolis is higher than that of Sphingomonas Polyaromaticivorans.By ultraviolet spectrum（UV）,infrared spectroscopy（IR）and gas chromatography-mass spectrometry（GC-MS）techniques,the liquid products of degradation were analyzed.The results show that the liquid products of degradation mainly include esters and amides.