气相色谱法测定苏籽中的a-亚麻酸

GC Determination of a-Linolenic in Perilla Seed

该文采用agilent极性19091X-233 HP-1 Bonded Polyethlene Glycol(30m×0.320mm,0.25μm)色谱柱,以外标法测定苏籽中α-亚麻酸含量。正己烷抽提苏籽中的α-亚麻酸,测定经氢氧化钾-甲醇皂化甲酯化后形成的α-亚麻酸甲酯的含量,直接得出苏籽中α-亚麻酸的含量。结果表明,α-亚麻酸甲酯标品的保留时间为22.785 min,α-亚麻酸甲酯的线性检测范围为0.01~10.00mg/m L,r=0.9981,方法回收率为96.1%~102.4%。

α-Linolenate was extracted with n-hexane from perilla seed,and methylified with 0.8 mol/L KOH-meth-noal solution.Then content of α-methyl linolenate,which could be translated to the content of α-linolenate,wasquantified with gas chromatographyusing agilent 19091 X-233 HP-1 Bonded Polyethlene Glycol(30 m × 0.320 mm,0.25μm).The retention time for α-methyl linolenate was 22.785 min.This method was appicable in a linear range of0.01-10.00 mg/m L with the correlation coefficient at 0.9981 and the average recovery for α-linolenate at 96.1% ~102.4%.