慢性阻塞性肺疾病患者血清中CCL18、CC16、IL-8的表达及临床意义

Serum CCL18,CC16,IL-8 in the Expression and Significance of Patients with Chronic Obstructive Pulmonary Disease

目的:观察慢性阻塞性肺疾病稳定期患者血清中趋化因子配体-18(CCL-18)、Clara细胞分泌蛋白(CC16)及白细胞介素-8(IL-8)的表达水平,探讨以上指标在COPD发病机制中的作用及临床意义。方法:选取83例COPD稳定期患者为病例组,78例健康体检人群为对照组。采用酶联免疫吸附(ELISA)法测定两组血清中的CCL18、CC16、IL-8水平,同时对两组受试对象行肺功能检查,比较上述指标在各组之间的差异,同时对COPD组CCL18、CC16、IL-8水平与肺功能第1秒用力呼气容积占预计值百分比(FEV1%pred)进行相关性分析。结果:病例组的CCL18、IL-8水平均显著高于对照组,CC16水平明显低于对照组,差异均有统计学意义(P<0.05)。病例组FEV1%pred显著低于对照组,差异有统计学意义(P<0.05)。病例组血清CCL18、IL-8与FEV1%pred均呈负相关,CC16与FEV1%pred呈正相关,相关系数分别为r=-0.591,r=-0.877,r=0.651,均有统计学意义(P<0.01)。结论:CCL18、CC16、IL-8在COPD气道及肺部炎症的发生发展、气流阻塞等病理变化中起重要作用,且与疾病的严重程度及预后相关。

Objective：To observe the serum of patients with chronic obstructive pulmonary disease in the chemokine ligand-18（CCL18）and Clara cell secretory protein（CC16）and interleukin-8（IL-8）expression levels of the above indexes in pathogenesis of COPD and its clinical significance.Methods：83 patients with stable COPD were selected as the case group,and 78 healthy people as control group.By enzyme-linked immunosorbent assay（ELISA）method for the determination of two groups of serum CCL18,CC16 and IL-8 levels,while two subjects underwent pulmonary function test,the difference between the indicators in each group,the COPD group of CCL18,CC16,IL-8 level and lung function FEV1 percentage of predicted value the percentage（FEV1%pred）correlation analysis.Results：CCL18 and IL-8 in the case group were significantly higher than those in the control group,the level of CC16 was significantly lower than that of the control group,the difference was statistically significant（P0.05）.The percentage of forced expiratory volume in the first second of the case group was significantly lower than that of the control group（FEV1%pred）,and the difference was statistically significant（P0.05）.Cases of serum CCL18,IL-8 and FEV1%pred were negatively correlated,CC16 was positively associated with FEV1%pred,the correlation coefficient was r=-0.591,r=-0.877,r=0.651,were statistically significant（P0.01）.Conclusion：CCL18,CC16 and IL-8 play an important role in the pathogenesis of airway inflammation and airway obstruction in COPD,and are related to the severity of the disease.