元宝枫种仁70%乙醇提取物的化学成分分析

Chemical Constituents of 70% EtOH Extract from the Kernel of Acer truncatum Bunge

目的:对新资源食品元宝枫种仁中的化学成分进行研究。方法:采用硅胶、聚酰胺、凝胶(Sephadex LH-20)柱色谱及重结晶等技术进行分离纯化,综合运用质谱和波谱学方法鉴定化合物结构。结果:从元宝枫种仁70%乙醇溶液提取物中分离鉴定了12种化合物,分别为二氢去氢二愈创木基醇-4'-O-β-D-葡萄糖苷(1)、1,2,3,6-四没食子酰基-O-β-D-吡喃葡萄糖(2)、1,2,3,4,6-五没食子酰基-O-β-D-吡喃葡萄糖(3)、槲皮素(4)、山柰酚-3-O-α-L-吡喃鼠李糖苷(5)、槲皮素-3-O-α-L-吡喃鼠李糖苷(6)、槲皮素-3-O-β-D-吡喃半乳糖苷(7)、槲皮素-3-O-α-L-吡喃阿拉伯糖苷(8)、阿夫儿茶素(9)、儿茶素(10)、表儿茶素(11)、胡萝卜苷(12)。结论:经Sci Finder检索发现,化合物1~12均为首次从元宝枫种仁中分离得到,其中化合物1、2、9~11为首次从槭树属植物中分离得到。

Objective：To study the functional components from the kernel of Acer truncatum Bunge as a new food resource.Methods：The chemical constituents were isolated and purified by sequential chromatographies on silica gel,polyamide and Sephadex LH-20 columns and recrystallization.Electrospray ionization mass spectrometry（ESI-MS）,1 H-NMR and 13 C-NMR spectroscopic analysis were employed for the structural elucidation.Results：A total of twelve compounds were obtained and they were chemically identified as（7 S,8 R）-urolignoside（1）,1,2,3,6-tetra-O-galloyl-β-D-pyranglucose（2）,1,2,3,4,6-penta-O-galloyl-β-D-pyranglucose（3）,quercetin（4）,kaempferol-3-O-α-L-rhamnopyranoside（5）,quercetin-3-O-α-L-rhamnopyranoside（6）,quercetin-3-O-β-D-galactopyranoside（7）,quercetin-3-O-α-L-arabinopyranoside（8）,（＋）-afzelechin（9）,（＋）-catechin（10）,（-）-epicatechin（11）,and daucosterol（12）.Conclusions：All these compounds were identified for the first time in the kernel of this plant,while compounds 1,2,and 9-11 were obtained for the first time from the genus Acer.