中国南方人群外周血microRNA-212表达与缺血性脑卒中的关联

Association between Blood MicroRNA-212 Expression and Ischemic Stroke in a Southern Chinese Population

目的探讨外周血miR-212表达水平与缺血性脑卒中风险的关联及其参与发病的潜在机制。方法选择44例缺血性脑卒中病例(病例组)及44例年龄、性别相匹配的对照人群(对照组),采用qRT-PCR检测外周全血miR-212的表达水平,非条件Logistic回归分析miRNA水平与缺血性脑卒中发生的关联,并通过生物信息学分析预测miR-212调控的下游信号通路。结果病例组外周血中miR-212表达水平显著高于对照组(P=0.012)。在校正年龄、性别等危险因素后,miR-212水平升高与缺血性脑卒中发病存在显著关联性[OR=1.62,95%CI为1.05~2.48,P=0.029]。miR-212表达水平与血小板数量呈负相关(r=-0.268,P=0.016)。生物信息学分析发现TGF-β、MAPK免疫炎症通路受到miR-212调控。结论外周血miR-212表达水平与缺血性脑卒中发生显著关联,其可能通过调控炎症参与发病。

Objective To investigate the association between blood miR-212 expression and is-chemic stroke（IS）,and to explore the potential mechanism for IS pathogenesis.Methods The ex-pression of miR-212 in peripheral blood was measured by qRT-PCR in 44 IS patients and 44 age-and sex-matched healthy controls.The association between miR-212 expression and IS occurrence was analyzed using multiple logistic regression analysis.Furthermore,the target genes of miR-212 was predicted using bioinformatics analysis.Results The expression of miR-212 in IS patients was significantly higher than that in healthy controls（P =0.012）.After adjustment for age,sex and other risk factors,the expression of miR-212 was significantly associated with the occurrence of IS（OR=1.62,95% CI =1.05-2.48,P =0.029）.Moreover,miR-212 expression was negatively correlated with platelet count（r=-0.268,P =0.016）.Bioinformatic analysis showed that TGF-β and MAPK pathways were regulated by miR-212.Conclusion The expression of miR-212 in pe-ripheral blood may be involved in IS through regulating inflammatory pathways.