刺葡萄皮醇提物和不同洗脱组分抗氧化成分及活性（英文）

Antioxidant Activity of Methanol Extract and Its Fractions from Spine Grape(Vitis davidii Fo?x.)Peel

以酸化甲醇为溶剂对刺葡萄皮进行超声波辅助提取,并用大孔树脂进行分离得到5种不同洗脱组分,研究刺葡萄皮醇提物和不同洗脱组分的活性成分及抗氧化能力。结果表明,刺葡萄皮含有较高含量的多酚(1.836 mg/g m_f)、黄酮(0.874 mg/g m_f)、VC(3.567 mg/g m_f)、丹宁(3.578 mg/g m_f)和花青素(2.970 mg/g m_f),其1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基清除能力、2,2’-二氮-双(3-乙基苯并噻唑-6-磺酸)二铵盐自由基(2,2’-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)radical,ABTS^+·)清除能力、铁离子还原能力(ferric reducing antioxidant power,FRAP)分别为22.325、17.595、26.487μmol TE/g m_f。在5种组分中以水解吸组分活性成分含量最高,其多酚、黄酮、VC、丹宁和花青素含量分别为0.876、0.116、1.577、1.576 mg/g m_f和1.330 mg/g m_f,其DPPH自由基、ABTS^+·清除能力和FRAP分别为3.636、10.109、13.415μmol TE/g m_f,相关性分析表明抗氧化活性与活性成分花青素、丹宁、VC含量呈强相关关系,表明抗氧化活性可能主要由这3种活性物质贡献。本研究结果表明,刺葡萄皮有潜力作为膳食补充抗氧化物质的主要来源。

In order to evaluate the antioxidant activity and components of spine grape peel, crude extract from spine grape peel was prepared by ultrasonic assisted extraction with acidified methanol as the extraction solvent and separated by macroporous resin adsorption to obtain five main fractions （I–V）. Spine grape peel showed high contents of total phenolics （1.836 mg/g mf）, total flavonoids （0.874 mg/g mf）, VC （3.567 mg/g mf）, total condensed tannins （3.578 mg/g mf） and total anthocyanins （2.970 mg/g mf） . The antioxidant activity of the methanol extract as evaluated by means of 1,1-diphenyl- 2-picrylhydrazyl （DPPH） assay, 2,2’-azino-bis （3-ethylbenzthiazoline-6-sulfonic acid （ABTS） assay and ferric reducing antioxidant power （FRAP） was 22.325, 17.595 and 26.487 μmol TE/g mf, respectively. Among 5 fractions, the watereluted fraction I contained the highest contents of phenolics, flavonoids, VC, tannin and anthocyanins （0.876, 0.116, 1.577, 1.576 and 1.330 mg/g mf, respectively） and presented the best antioxidant activity （3.636, 10.109 and 13.415 μmol TE/g mf for DPPH radical scavenging activity, ABTS＋· scavenging activity and FRAP, respectively）. Significant correlations were observed between antioxidant activity and anthocyanins, tannin and VC contents, which suggested that these compounds might be the major antioxidants contributing to the observed activity. Therefore, spine grape peel is a promising source of antioxidants and can be used as a dietary supplement for health promotion.