紫薯提取物对秀丽隐杆线虫抗氧化作用的影响

Antioxidant Effect of Purple Sweet Potato Extract in Caenorhabditis elegans

目的:以秀丽隐杆线虫为对象,研究紫薯提取物(purple sweet potato extract,PSPE)抗氧化作用及其可能的作用机制。方法:将秀丽隐杆线虫饲喂于含有不同质量浓度(70、140、280μg/mL)PSPE的线虫生长培养基,研究PSPE对线虫寿命、抗氧化酶活力以及相关基因表达水平、急性氧化应激的影响。结果:PSPE能明显延长秀丽隐杆线虫的寿命,提高超氧化物歧化酶和过氧化氢酶活力,上调daf-16、ctl-1、sod-3、sir-2.1基因表达水平,下调age-1、daf-2基因表达水平,同时延长在H_2O_2、胡桃醌及百草枯氧化应激的存活时间。结论:PSPE能够有效延缓秀丽隐杆线虫的衰老、抑制脂褐素累积、提高抗氧化酶活力及抗氧化基因表达水平,增强对H_2O_2、胡桃醌及百草枯急性氧化应激的抵抗能力。

Objective： We used Caenorhabditis elegans as a model organism to study the anti-aging effect of purple sweet potato extract （PSPE） and to elucidate its possible mechanism. Methods： C. elegans were fed with NGM culture medium containing different concentrations （70, 140, 280 μg/mL） of PSPE, and the effect of PSPE on the lifespan, the activity of antioxidant enzymes, the expression of related genes and the resistance to acute oxidative stress were determined. Results： PSPE could markedly prolong the lifespan of C. elegans, increased the activity of antioxidant enzymes such as superoxide dismutase （SOD） and catalase （CAT）, up-regulated the gene expression levels of daf-16, ctl-1, sod-3 and sir-2.1, and down-regulated the expression levels of age-1 and daf-2. At the same time, it could extend the survival time under H2O2, juglone and paraquat stress. Conclusion： PSPE could slow down the aging process of C. elegans, increase the activity of antioxidant enzymes, promote the expression of antioxidant genes, and enhanced the resistance to oxidative stress induced by H2O2, juglone or paraquat.