GMA诱导16HBE恶性转化细胞中LncRNAEMX2OS的表达及意义

Expression of LncRNA EMX2OS during glycidyl methacrylate-induced malignant transformation of 16HBE cells

目的:探讨Lnc RNA EMX2OS在甲基丙烯酸环氧丙酯(GMA)诱导的16HBE恶性转化细胞中的表达变化及意义。方法:取GMA诱导的第30代16HBE恶性转化细胞及DMSO对照组细胞,采用高通量Lnc RNA芯片比较两组样本表达谱的差异,并用生物信息学方法筛选出Lnc RNA EMX2OS及其靶向基因EMX2,采用实时荧光定量PCR(q PCR)分析16HBE恶性转化细胞中Lnc RNA EMX2OS和EMX2 m RNA的表达水平,并与对照组细胞比较。结果:Lnc RNA芯片结果显示,与DMSO对照组细胞相比,GMA诱导的16HBE恶性转化细胞中Lnc RNA EMX2OS上调6.01倍;q PCR结果显示,相对于对照细胞,16HBE恶性转化细胞中Lnc RNA EMX2OS表达上调3.37倍,EMX2 m RNA上调1.88倍(P<0.05)。EMX2 m RNA和Lnc RNA EMX2OS表达的变化趋势一致。结论:GMA可以诱导16HBE细胞Lnc RNA EMX2OS表达上调,Lnc RNA EMX2OS可作为GMA诱导的16HBE恶性转化细胞中相关特异分子标志之一。

OBJECTIVE： To investigate the expression of long non-coding RNA EMX2OS （LncRNA EMX2OS） during glycidyl methacrylate （GMA）-induced malignant transformation of 16HBE cells. METHODS：Malignantly transformed 16HBE cells （induced by exposure to 8 μg/mL GMA） and solvent control cells （exposed to DMSO） at around the 30th generation were harvested. High throughput LncRNA microarrays were used to detect differences in expression profile of LncRNAs between the two groups of cells. Changes in LncRNAs were screened through strategies such as fold changes and neighboring genes analyses. Real-time fluorescence quantitative PCR （qPCR） and gene chip were applied to measure the expression levels of LncRNA EMX2OS and EMX2,respectively. RESULTS：Based on the result of LncRNA microarrays,expressions of LncRNA EMX2OS was up-regulated by 6.01 fold in the transformed cells campared to the control cells. In addition, qPCR analyses showed that LncRNA EMX2OS increased 3.37 fold and EMX2 increased 1.88 fold change （P〈0.05）. The trends of increase in EMX2 and EMX2OS trends were identical. CONCLUSION：GMA induced significant increase in expression of LncRNA EMX2OS of 16HBE cells and the expression can be considered as a specific biomarker which is involved in GMA-induced malignant transformation 16HBE cells.