红花草莓的组织培养与快繁技术研究

Tissue culture and rapid propagation technique of red-flowered strawberry

以红花草莓叶片为外植体,通过筛选诱导愈伤组织、不定芽及壮苗、生根的培养基,建立一套实用且易推广的红花草莓组培快繁技术体系。结果表明:在愈伤组织的诱导过程中TDZ的诱导效果优于6-BA,TDZ与NAA配合使用效果优于与IBA的组合。6-BA浓度为0.5 mg·L^(-1)时不定芽诱导率高达86.6%。低浓度的6-BA和8 g·L^(-1)的琼脂更有利于壮苗培养,NAA比IBA更有利诱导生根。综上述,最适红花草莓愈伤组织的诱导培养基为MS+1.0 mg·L^(-1)TDZ+0.5 mg·L^(-1)NAA+30 g·L^(-1)蔗糖+7 g·L^(-1)琼脂;最适不定芽分化的培养基为MS+0.5 mg·L^(-1)6-BA+0.1 mg·L^(-1)NAA+30 g·L^(-1)蔗糖+7 g·L^(-1)琼脂;最适壮苗培养基为MS+0.1 mg·L^(-1)6-BA+0.1 mg·L^(-1)NAA+30 g·L^(-1)蔗糖+8g·L^(-1)琼脂;最适生根培养基为MS+0.5mg·L^(-1)NAA+30 g·L^(-1)蔗糖+8 g·L^(-1)琼脂。试管苗移栽生长20 d后,成活率高达93%,且后期草莓苗生长壮健。此体系的建立为优质红花草莓种苗大规模生产提供了科学依据和技术支持。

We used leaves of red-flowered strawberry as materials,by selecting the best medium for the callus induction,adventitious bud induction and root growth to establish a rapid propagation technique system of red-flowered strawberry. The results showed that TDZ was better than 6-BA in the induction of callus,and TDZ and NAA combination had better effects than TDZ and IBA. When the concentration of 6-BA was 0.5 mg·L-1,the inductivity of adventitious bud reached 86.6%. The low 6-BA concentration and 8 g·L-1 agar were benefical for seedling growth. NAA was better than IBA in the rooting inducing. So the optimum medium for callus inducing was： MS ＋ 1.0 mg·L-1 TDZ ＋ 0.5 mg·L-1 NAA ＋ 30 g·L-1 sucrose ＋ 7 g·L-1 agar; the optimum medium for adventitious bud inducing was MS ＋ 0.5 mg·L-16-BA ＋ 0.1 mg·L-1 NAA ＋ 30 g·L-1 sucrose ＋ 7 g·L-1 agar; the optimum medium for seedling growthing was MS ＋ 0.1 mg·L-16-BA ＋ 0.1 mg·L-1 NAA ＋ 30 g·L-1 sucrose ＋ 8 g·L-1 agar; the optimum medium for rooting was MS ＋ 0.5 mg·L-1 NAA ＋ 30 g·L-1 sucrose ＋ 8 g·L-1 agar. The tissue culture seedlings grew well and the living ratio reached 93% after planted for 20 d. The establishment of rapid propagation technique system of red-flowered strawberry provides scientific and technological support for large-scale production of high quality red-flowered strawberry seedlings.