基于PrimerHunter的多重PCR引物设计系统的构建与应用

The Development and Application of Multiplex PCR Primers Design System Based on PrimerHunter

PrimerHunter是一款多重PCR引物设计软件,该软件能够高效地设计出具有特异性的多重引物,但是只能在Linux系统的命令行中运行,而且没有考虑引物二聚体的问题。针对这些问题,文章建立了基于PrimerHunter的多重PCR引物设计系统,实现了PrimerHunter的界面化,完成了C/S模式向B/S模式的转换,同时增加了原软件不具备的引物二聚体检测模块,能够有效检测引物对之间的二聚体情况,为相关生物学领域研究提供了一种高效的多重PCR引物设计工具。

PrimerHunter is a multiplex PCR design software,which can efficiently design multiplex primers specificity,but it only runs in the command line of the Linux system,and it doesn＇t take into consideration the problem of primer dimers.To remedy these defects,the authors of this paper developed a multiplex PCR primers design system based on PrimerHunter,turning PrimerHunter into interface,changing from C/S mode to B/S mode,and adding a new primer dimmers detect module.It can effectively detect the dimmers between the primer pairs and function as an effective tool for the designing of multiplex PCR primers in the related field of biology.