流式细胞学技术检测急性髓细胞白血病患者外周血淋巴细胞亚群的效果分析

Effect of flow cytometry on detection of peripheral blood lymphocyte subsets in patients with acute myeloid leukemia

目的分析流式细胞学技术检测急性髓细胞白血病患者外周血淋巴细胞亚群的变化的效果。方法选取108例急性髓细胞白血病患者为白血病组,另选取108例健康人员作为健康组,采用Multi SET免洗淋巴细胞亚群试剂盒,运用美国BD公司生产的流式细胞仪CD45/SSC设门检测10 000个细胞,采用FACS Diva软件对细胞免疫表型及表达强度进行分析。结果白血病组患者的CD3、CD4、CD_4^+/CD_8^+、CD_(16)^+/CD_(56)^+均显著低于健康组(P<0.05),CD_8^+、CD_(19)^+均显著高于健康组(P<0.05)。细胞CD_(33)、CD_(38)、Cyt-MPO抗原高表达数比例较高,分别占总数的72.5%、70.4%、67.5%;其次为CD_(15)、CD_(64),分别占总数的38.0%、32.5%。高表达CD_(33)和CD_(64)及Cyt-MPO表达均呈显著正相关(P<0.05),但是和CD_(15)表达无相关性(P>0.05)。结论流式细胞学技术检测急性髓细胞白血病患者外周血淋巴细胞亚群,能够对其变化规律有一个清晰的了解。

ObjectiveTo analyze the effect of flow cytometry on detection of peripheral blood lymphocyte subsets in patients with acute myeloid leukemia. MethodsA total of 108 patients with acute myeloid leukemia were selected as leukemia group, and 108 healthy people were selected as control group. MultiSE lymphocyte subsets regents and flow cytometer （CD45/SSC, America） were used to detect 10 000 cells, and FACS Diva software was used to analyze the cellular immunophenotype and expression intensity. ResultsIn the leukemia patients, the CD3, CD4, CD4^＋/CD8^＋, CD16^＋/CD56^＋ were significantly lower than normal people, while CD8^＋, CD19^＋ were significantly higher （P〈0.05）. Expression of antihelion of cell CD33, CD38 and Cyt MPO were high, respectively accounting for 72.5%, 70.4% and 67.5%, and followed by CD15, CD64, which accounting for 38% and 32.5% respectively. High expressions of CD33, CD64 and Cyt MPO all showed positive correlations （P〈0.05）, but was not related with expression of CD15 （P〉0.05）. ConclusionFlow cytometric analysis of peripheral blood lymphocyte subsets in patients with acute myeloid leukemia can provide a clear understanding of the change of lymphocyte subsets.