卵巢癌血清中循环microRNA-7的表达及其与临床病理特征相关性分析

Expression of circulating microRNA-7 in serum of patients with ovarian cancer and its correlation with clinicopathological features

目的检测卵巢癌患者血清中microRNA-7表达水平,分析其与卵巢癌临床各病理特征相关性。方法荧光定量PCR检测卵巢癌患者、卵巢良性病者及健康体检者血清中microRNA-7的相对水平,并对卵巢癌各不同临床参数类别中microRNA-7表达水平进行统计学分析。结果卵巢癌血清中microRNA-7相对表达水平显著低于卵巢良性疾病组和健康体检者,差异有统计学意义(P<0.05)。临床Ⅲ期+Ⅳ期卵巢癌患者microRNA-7水平显著低于Ⅰ期+Ⅱ期,淋巴结转移者显著低于未转移者,中低分化者显著低于高分化者;CA125浓度≥35 U/ml组,其microRNA-7水平显著低于CA125浓度<35 U/ml组,差异均有统计学意义(P<0.05)。结论 microRNA-7在卵巢癌中低表达,与临床分期、淋巴结转移及分化程度亦相关,可作为卵巢癌诊断的潜在靶标。

Objective To detect the expression of serum microRNA-7 in patients with ovarian cancer,and analyze its correlation with the clinical pathological features of ovarian cancer. Methods The relative levels of microRNA-7 were detected by fluorescent quantitative PCR in the patients with ovarian cancer,benign ovarian disease and healthy controls,and the expressions of microRNA-7 in ovarian cancer with different clinical parameters were statistically analyzed in the category.Results The serum microRNA-7 expression in patients with ovarian cancer was significantly lower than that of the benign ovarian group and the healthy controls,with the differences statistically significant（ P〈0. 05）; the microRNA-7 levels of Ⅲ ＋Ⅳ stage in ovarian cancer patients were lower than that of Ⅰ ＋ Ⅱ period,and the concentrations of microRNA-7 in lymph node metastasis patients were lower than those without metastasis; that in patients with low differentiation were significantly lower than those with high differentiation; the patients with CEA≥35 U/ml had higher microRNA-7 concentration than the patients with CEA 35 U/ml,with the differences statistically significant（ P〈0. 05）. Conclusion The low expression of microRNA-7 in ovarian cancer is correlated with clinical stage,lymph node metastasis and differentiation,which may be a potential target for the diagnosis of ovarian cancer.