摘要
Mitochondria and lysosomes are essential cellular organelles in most eukaryotic cells by playing the physiological roles to support the normal functions of cells, as well as the life of the whole body. To date,small-molecule fluorescent probes have been considered as one of the vital tools for monitoring and visualizing multiple biological analytes. This review summarized the recent advances in small-molecule two-photon fluorescent probes for metal ions, reactive oxygen species（ROS） and reactive sulfur species（RSS）, and changes inside micro-environment（e.g., p H, viscosity and polarity） in mitochondria and lysosomes, or served as mitotracker and lysotracker with the assistance of two-photon microscopy.
Mitochondria and lysosomes are essential cellular organelles in most eukaryotic cells by playing the physiological roles to support the normal functions of cells, as well as the life of the whole body. To date,small-molecule fluorescent probes have been considered as one of the vital tools for monitoring and visualizing multiple biological analytes. This review summarized the recent advances in small-molecule two-photon fluorescent probes for metal ions, reactive oxygen species（ROS） and reactive sulfur species（RSS）, and changes inside micro-environment（e.g., p H, viscosity and polarity） in mitochondria and lysosomes, or served as mitotracker and lysotracker with the assistance of two-photon microscopy.
基金
supported by the National Natural Science Foundation of China (Nos. 21778001, 21372005)
the Anhui Provincial Natural Science Foundation (No. 1608085MB39)
the Natural Science Foundation of Education Department of Anhui Province (No. KJ2015A047)
the 211 Project of Anhui University
