加味水陆二仙丹对2型糖尿病大鼠肝脏脂肪变性的影响

Effects of modified Shuilu Erxian Dan on liver steatosis in rats with type 2 diabetes

目的探讨加味水陆二仙丹对实验性糖尿病大鼠血脂的影响,以及对肝脏的保护作用。方法采用高脂高热量饮食结合一次性腹腔注射1%链脲佐菌素(STZ)溶液35 mg/kg,建立2型糖尿病SD大鼠模型,随机分为模型组、罗格列酮组、中药治疗组,每组8只,另取8只正常大鼠作为对照,正常组给予基础饲料喂养,其余组给予高脂高热量饲料饲养,药物干预4周后,观察并比较各组大鼠血脂、血糖、胰岛素水平及肝组织形态学变化。结果与模型组比较,加味水陆二仙丹能显著降低大鼠肝重指数、TC、LDL-C、TG、血清胰岛素水平、肝细胞脂肪变程度,差异均有统计学意义(P<0.05)。结论加味水陆二仙丹可在一定程度上缓解非酒精性脂肪性肝病(NAFLD)病理学改变,可能与其能降低血脂水平有关,改善高胰岛素血症可能是加味水陆二仙丹防治NAFLD的作用机制之一。

Objective To explore the effects of modified Shuilu Erxian Dan on blood fat in experimental diabetic rats and its protective effect on liver.Methods SD diabetic rat model were induced by high-fat high calories diet combined intraperitoneal injection once of 1% streptozotocin（STZ solution） with 35 mg/kg,and were randomly divided into model group,rosiglitazone group,and herbal medicine treatment group of 8 rats each.Another 8 normal rats were used as the control group.The control group was given with basic feeding,and the other groups were fed with high fat and high calorie diet.After 4 weeks of feeding and intervention,the changes of blood fat,blood sugar,insulin level and liver tissue morphology of all groups were observed and compared.Results Compared with the model group,modified Shuilu Erxian Dan could significantly decreased the liver weight index,TC,LDL-C,TG,insulin levels,hepatic steatosis,and the differences were statistically significant（P〈0.05）.Conclusion Modified Shuilu Erxian Dan can alleviate the pathological and histological changes in NAFLD in a certain extent,which probably is related to its function in decreasing the serum lipid level.The improvement of insulin resistance could be one of the mechanisms of modified Shuilu Erxian Dan in the prevention and treatment for NAFLD.