高原地区不同海拔下大鼠SAP相关肾损伤变化的研究

Severe acute pancreatitis-related renal injury in rats at different altitudes

目的研究高原地区不同海拔下大鼠重症急性胰腺炎(severe acute pancreatitis,SAP)相关肾损伤的变化情况.方法在不同海拔不同时间点通过胰腺被膜下推注4.5%牛磺胆酸钠建立大鼠SAP模型,对照组(F组)开腹后轻触胰腺建立对照组模型.分别于相应时间点采大鼠腹主动脉血、胰腺组织,检测血淀粉酶(amylase,AMY)、尿素氮(blood urea nitrogen,BUN)、肌酐(creatinine,Cr)值,显微镜下观察胰腺组织HE染色切片,给胰腺组织评分,进行统计学分析.结果血清AMY、Cr、BUN值的变化:各SAP组血清AMY、Cr、BUN值在同一海拔不同时间点比较,差异有统计学意义(P<0.05),各SAP组与F组血清AMY、Cr、BUN值比较,差异有统计学意义(P<0.05),SAP各组血清AMY、Cr、BUN值在不同海拔下同一时间点比较,差异有统计学意义(P<0.05),不同海拔的F组血清AMY、Cr、BUN值比较,差异有统计学意义(P<0.05);胰腺组织病理评分:各SAP组胰腺组织评分在同一海拔不同时间相比较,差异有统计学意义(P<0.05),随着海拔的逐渐升高,组织病理评分在相同时间点比较,差异有统计学意义(P<0.05).结论高原低氧环境加重胰腺炎合并肾损伤疾病的发生发展过程.

AIM To compare severe acute pancreatitis（SAP）-induced renal injury in rats at different altitudes. METHODS SAP was induced in rats by injecting 4.5% sodium taurocholate into the pancreas at different time points. Control rats underwent a sham operation（touching the pancreas only after opening the abdominal cavity）. Blood samples were collected from the abdominal aorta at different time points to determine serum levels of amylase（AMY）, creatinine（Cr）, and blood urea nitrogen（BUN）. Pancreas tissues were collected and stained with HE to perform histopathological analysis. RESULTS The levels of serum AMY, Cr, and BUN were significantly higher in the SAP group than in the control group（P 〈 0.05）. The levels of serum AMY, Cr, and BUN in the SAP group differed significantly at the same time point at different altitudes（P 〈 0.05） or at different time points at the same altitude（P 〈 0.05）. There was a significant difference in pancreatic histopathological score between the two groups（P 〈 0.05）. The histopathological score in the SAP group differed significantly at the same time point at different altitudes（P 〈 0.05） or at different time points at the same altitude（P 〈 0.05）. CONCLUSION The development and progression of renal injury are aggravated at high altitudes.