携MAGE靶向金纳米粒光声及超声显像黑色素瘤的实验研究

MAGE-targeted gold nanoparticles for enhancing photoacoustic and ultrasound imaging on melanoma

目的制备抗黑色素瘤相关抗原(MAGE)抗体偶联的载金纳米棒靶向纳米分子探针,探讨其对体外恶性黑色素瘤细胞的靶向性,观察其体外光声成像效果。方法采用双乳化法制备包裹金纳米棒和液态氟碳的聚乳酸-羟基乙酸共聚物(PLGA)纳米粒,碳二亚胺法连接靶向MAGE的单克隆抗体,制备高分子多功能靶向PLGA纳米分子探针,检测其一般物理特性、MAGE抗体与纳米粒的连接情况及其体外寻靶能力,并观察体外光声成像效果。结果成功制备的靶向载金纳米棒多功能纳米分子探针平均粒径(336.40±27.46)nm,平均Zeta电位(-4.34±4.9)m V。MAGE抗体与纳米粒有效连接。体外寻靶能力实验显示黑色素瘤B16细胞株周围有大量靶向纳米探针环绕;光声成像显示随金纳米粒含量增加,其光声信号逐渐增强。结论 MAGE抗体偶联金纳米棒纳米分子探针制备成功,对体外恶性黑色素瘤细胞具有较好的生物活性,细胞靶向性较好,且体外光声成像有明显增强信号,可作为光声成像造影剂,进一步行肿瘤靶向光热治疗。

Objective To investigate the ability of targeting in vitro melanoma of nanoparticle probe loaded with gold nanorod and its consequence of photoacoustic imaging in vitro.Methods The polymeric multifunctional nanoparticles probe loaded with gold nanorod and liquid perfluorocarbon,and connected with MAGE-antibody targeting melanoma was prepared by double emulsion method and carbodiimide method.General physical property,the condition of MAGE-antibody connected with nanoparticles and the ability of targeting of the probe were tested.The different concentration of nanoparticles was imaged by photoacoustic.Results The targeting polymeric multifunctional nanoparticles probes loaded with gold nanorod were prepared successfully with average particle diameter of（336.40±27.46）nm and Zeta potential of（-4.34±4.90）m V.MAGE-antibody was successfully connected with the nano probes.There were massive targeting nano probes surrounded the melanoma cell strains B16 in the targeting group in vitro.The photoacoustic signal of the nano probes was enhanced with the increase of gold nanorod concentration in photoacoustic experiment in vitro.Conclusion The prepared polymeric multifunctional nanoparticles probe loaded with gold nanorod and connected with MAGE-antibody targeting melanoma can be used as a photoacoustic contrast agent,which can inhibit the proliferation of melanoma by targeting photothermal therapy.