HPLC法同时测定藿香正气系列制剂中6种成分的含量

Simultaneous Determination of Six Components in Huoxiang Zhengqi Series Preparations by HPLC

目的:建立HPLC法同时测定藿香正气系列制剂中橙皮苷、欧前胡素、异欧前胡素、厚朴酚、和厚朴酚、苍术素的含量。方法:采用Waters C_(18)色谱柱(250 mm×4.6 mm,5μm),流动相A为乙腈,流动相B为0.5%的冰醋酸水溶液,进行梯度洗脱;流速:1.0 ml·min^(-1),检测波长:290 nm,柱温:40℃,进样量:10μl。结果:藿香正气系列制剂中的橙皮苷、欧前胡素、异欧前胡素、厚朴酚、和厚朴酚和苍术素6种成分的线性关系良好(r≥0.999 3),平均加样回收率分别为97.0%,95.0%,94.3%,97.6%,97.3%,93.2%,RSD分别为2.25%,2.60%,2.51%,1.67%,1.78%,2.50%(n=9)。结论:该方法重复性好、精密度高、适用性强、简便快捷,可用于藿香正气系列制剂的质量控制。

Objective：To establish an HPLC method for the determination of hesperidin, imperatorin, isoimperatorin, magnolol, honokiol and atractylodin in Huoxiang Zhengqi series preparations. Methods：An HPLC method was used with a Waters C18 chromato-graphic column （250 mm × 4. 6 mm, 5 μm）. The mobile phase consisted of acetonitrile-0. 5% glacial acetic acid aqueous solution re-spectively for the mobile phase A and B with gradient elution. The flow rate was 1. 0ml·min-1;the detection wavelength was 290 nm;the column temperature was set at 40℃;the sample size was 10μl. Results：The linearity ranges of hesperdin, imperatorin, isoimper-atorin, magnolol, honokiol and atractylodin showed good linear relationships（r≥0.9993）. The average recovery was 97.0%, 95. 0%,94. 3%,97. 6%,97. 3% and 93. 2%, respectively. The RSD was 2. 25%,2. 60%,2. 51%,1. 67%,1. 78% and 2. 50%（ n=9）, respectively. Conclusion：The method is simple and quick with good reproducibility, high precision and promising applicabili-ty, which can be used for the quality control of Huoxiang Zhengqi series preparations.