整体柱HPLC法测定三七总皂苷中的5种皂苷成分

Simultaneous Quantitative Determination of Five Saponins in Notoginseng Total Saponins by HPLC with a Monolithic Column

目的:建立三七总皂苷中三七皂苷R1、人参皂苷Rg_1、人参皂苷Re、人参皂苷Rb_1及人参皂苷Rd含量的HPLC快速测定方法。方法:采用Merck Chromolith Performance RP-18e整体柱(100 mm×4.6 mm,2μm),乙腈-水为流动相,流速/流动相双梯度洗脱,检测波长为203 nm。结果:5种皂苷成分在20 min内完全分离,各成分在测定范围内线性关系良好(r≥0.999 8),平均加样回收率为98.6%~100.4%,RSD均<2.1%(n=6)。结论:该法高效准确,可用于三七总皂苷的质量控制。

Objective：To establish a rapid HPLC method for the quantitative determination of 5 saponins （ notoginsenoside R1 , ginsenoside Rg1 ,ginsenoside Re,ginsenoside Rb1 and ginsenoside Rd） in Notoginseng total saponins using a monolithic column. Meth-ods：The analysis was performed on a Merck Chromolith Performance RP-18e column （100 mm × 4. 6 mm,2μm） with gradient elution of acetonitrile and water. The detection wavelength was set at 203 nm. Results：Satisfactory separation of all analytes was obtained in 20 min. All calibration curves showed good linearity within the testing ranges （r≥0.9998）. The average recoveries were between 98. 6% and 100. 4%. The RSDs were less than 2. 1% （n=6）. Conclusion：The method is efficient and accurate for the quality con-trol of Notoginseng total saponins.