消疕汤对大鼠银屑病样皮损处病理改变的影响

The Effect of Xiaobi Decoction on Pathologic Changes of Rat Psoriasis-like Skin Lesions Expression

目的:将大鼠诱导成银屑病样皮损模型,并对其进行治疗,证实消疕汤对大鼠银屑病样皮损处病理改变有影响,为消疕汤的临床应用提供实验室依据。方法:(1)将50只SD大鼠(雌雄各半)随机分成五组,即空白组、造模组、造模后对照组、消疕汤组、阿维A胶囊组,除空白组正常饲养外,余四组用心得安乳液涂抹大鼠双侧耳廓皮肤,两周后取下造模组大鼠双侧耳廓皮肤,显微镜下观察病理改变见明显银屑病样皮损改变后提示造模成功;(2)造模成功后消疕汤组和阿维A胶囊组分别予相应药物灌胃治疗,两周后取下各组大鼠双耳组织,切片、HE染色观察各组病理差异;(3)用Baker评分法对各组切片进行评分并进行统计学处理。结果:(1)除空白组以外,余四组在涂抹心得安乳剂2周后耳廓组织相继出现局部红肿、鳞屑增多、刮之易落、皮肤萎缩等症状,造模组送检表现出角化过度、角化不全等典型银屑病样病理改变,Baker评分统计学处理结果示造模组与空白组相比较(P<0.05)组间有统计学差异,提示造模成功;(2)经消疕汤和阿维A胶囊治疗后,其耳部组织的角化过度、角化不全等病理情况得到明显改善,统计学处理结果示消疕汤组和阿维A胶囊组Baker评分与造模组相比较P<0.05,差异有统计学意义,提示治疗有效;(3)消疕汤组与阿维A胶囊组评分相比P=0.116>0.05,差异无统计学意义,提示消疕汤和阿维A胶囊均有抑制角化过度、角化不全的作用,且疗效无明显差异。结论:(1)用心得安乳液涂抹制造银屑病样皮损的造模方法可行;(2)消疕汤和阿维A胶囊对银屑病样皮损处病理改变治疗均有效。

Objective： To treat rats with induced psoriasis-like skin lesions so as to confirm whether Xiaobi decoction has effect on pathologic changes of rat psoriasis-like skin lesions and provide an experimental basis for the clinical application of Xiaobi decoction. Methods： At first, 50 SD rats with half males and half females were randomly divided into five groups, namely the control group, the modeling group, the control group after modeling, the Xiaobi decoction group and the acitretin capsule group. Propranolol emulsion was applied on the auricle skin of two ears in all rats except the control group, rats in the control group were fed normally. After two weeks, the auricle skins of two ears of rats in the modeling group were collected, and the pathologic changes under microscope showed obvious psoriasis-like skin lesions, indicating that the modeling was succeeded. Secondly, after success of modeling, rats in the Xiaobi decoction group and the acitretin capsule group were treated with intragastric administration of corresponding drugs respectively. The auricle skin tissues of two ears of rats of each group were collected, sectioned, and stained by HE to observe their pathological changes. Finally the sections of each group were scored using Baker score and analyzed statistically. Results： Except the control group, all other four groups exhibited local swelling, scales increasing, easy falling when scaling, skin atrophy and other symptoms in auricle tissues after two weeks of using propranolol emulsion. Typical psoriasis-like pathological changes like hyperkeratosis and parakeratosis are reported in submitted samples from the modeling group. The results of Baker scores demonstrated statistically significant differences between the modeling group and the control group （P〈0.05）, which indicated the sUccessful modeling. After the treatment using Xiaobi decoction and acitretin capsules, in auricle tissues the pathological conditions including hyperkeratosis, parakeratosis were significantly improved. Results showed statistically significant differences （P〈0.05） in the Xiaobi decotion group and the actitretin capsule group compared with modeling group, thus confirming the treatment effectiveness. The difference in scores between the Xiaobi decoction group and acitretin capsule group was not statistically significant （P=0.116）, indicating that both Xiaobi decoction and acitretin capsules can inhibit hyperkeratosis and parakeratosis without significant difference in efficacy. Conclusions： It is feasible to build psoriasis-like skin lesion models by applying propranolol emulsion. Both Xiaobi decoction and acitretin capsule are proved to be effective in improving the pathological symptoms for psoriasis-like skin lesions.