丽影和红韵蝴蝶兰快速繁殖试验

Micropropagation of Phalaenopsis ‘Liying' and ‘Hongyun'

以花梗腋芽为材料,研究了蝴蝶兰新品种丽影和红韵的快速繁殖技术。结果表明,消毒时间对营养芽诱导率有显著影响,0.1%Hg Cl2消毒8 min的红韵蝴蝶兰营养芽诱导率最高,激素对营养芽诱导率影响不显著。6-BA浓度、有机附加物和芽数对丛生芽增殖影响显著,当6-BA浓度为8.0 mg/L时,丽影和红韵芽增殖系数最高,分别为2.31和2.22;添加椰汁更有利于减轻褐变,提高增殖系数;单芽接入的增殖系数最高。激素对生根率无显著影响,但对平均根数和苗高有显著影响,在含6-BA 0.1 mg/L+NAA 1.0 mg/L的培养基上试管苗株高最高,平均根数最多。以松树皮为基质,丽影和红韵蝴蝶兰试管苗移栽成活率分别为91.58%和87.27%。

Micropropagation of new cultivars of Phalaenopsis ‘Liying' and Phalaenopsis ‘Hongyun' was investigated by using of peduncle as explant.The results indicated that disinfection time had significant effect on the induction rate of vegetative shoot,after disinfected with 0.1% Hg Cl2 for 8 minutes,the induction rate of vegetative bud was the highest,while exogenous hormones had no significant effect on the induction rate of Phalaenopsis‘Hongyun'.The concentration of 6-BA,organic additives and the number of bud in an inoculums had significant influence on bud proliferation.At the concentration of 6-BA being 8 mg/L,the proliferation coefficients of Phalaenopsis ‘Liying' and Phalaenopsis ‘Hongyun' were the highest,accounting for 2.31 and 2.22,respectively.Supplying coconut milk in proliferation medium was more beneficial for alleviating the browning and enhancing the proliferation coefficient compared with adding potato or banana.Single bud as an inoculum favored bud proliferation and enhanced the multiplication coefficient.Exogenous hormone had little effect on the root induction rate,but had significant effect on the mean number of root and height of plantlet.Seedling height of Phalaenopsis ‘Hongyun' and Phalaenopsis ‘Liying' was the highest and the mean number of root was the most on modified 1/2 MS medium with 6-BA 0.1 mg/L + NAA 1 mg/L.By using pine bark as culture substrate,the survival rates of Phalaenopsis ‘Liying' and Phalaenopsis ‘Hongyun' were 91.58% and 87.27%,respectively.