XIAP在尖锐湿疣组织中的表达及其与HPV分型的相关性

Expression of XIAP in condyloma acuminatum and its correlations with HPV subtypes

目的探讨X连锁凋亡抑制蛋白(XIAP)在尖锐湿疣组织中的表达及其与人乳头瘤病毒感染的关系。方法免疫组织化学方法分别检测尖锐湿疣和正常包皮组织中XIAP的表达,采用实时聚合酶链式反应体外扩增和反向点杂交相结合的DNA芯片技术对尖锐湿疣组织进行基因分型检测。结果正常包皮组、尖锐湿疣未复发组和复发组中XIAP的阳性表达率依次升高(分别是26.67%、53.49%和79.31%),3者之间两两比较,差异有统计学意义(P<0.05);XIAP在高危型HPV组与正常对照组的表达比较,差异有统计学意义(P<0.05),高危型HPV组高于对照组;在高危型与低危型组间、低危型与正常对照组间的表达比较,差异无统计学意义(P>0.05);XIAP在不同年龄及性别患者间的表达比较,差异无统计学意义(P>0.05);而患者不同的病程及复发次数,XIAP阳性表达率比较,差异有统计学意义(P<0.05)。结论尖锐湿疣组织中XIAP表达上调可能是其发生和复发的重要原因,XIAP在尖锐湿疣的恶性转化过程中可能起促进作用,对判断患者病情预后具有一定的参考价值。

Objective To investigate the roles of X-linked inhibitor of apoptosis protein （XIAP） in condyloma acuminatum and to study the relationships between XIAP and Human Papilloma Virus （HPV） subtypes. Methods The immunohistochemical method was adopted to detect the expression of XIAP in condyloma acuminatum and healthy prepuce tissues. HPV genotypes were detected by PCR and gene chip in all condyloma acuminatum tissues. Results The expression rate of XIAP was gradually increased from normal prepuce, nonrecurrent condyloma acuminatum to recurrent condyloma acuminatum tissues （26.67%, 53.49% and 79.31% respectively） with signifcant differences （P 〈 0.05）. Futhermore, the XIAP expression level in the high-risk HPV group was higher than that in the normal prepuce （66.67% vs. 26.67%, P 〈 0.05）; however, it was similar between the high-risk and low-risk HPV groups as well as low-risk HPV group and normal prepuce （P 〉 0.05）. The positive expression rate of XIAP was statistically different between the patients with different disease courses and recurrent times （P 〈 0.05）, but not between those groups with different sex and age （P 〉 0.05）. Conclusions Elevated expression of XIAP may play an important role in the pathogenesis and recurrence of condyloma acuminatum. It may promote the malignant transformation of condyloma acuminatum, and serve as an effective and objective marker in detection and mornitoring of condyloma acuminatum.