精神分裂症免疫球蛋白N-糖链半乳糖基化水平及临床意义

Galactose base level of N-sugar chain and clinical significance in immunoglobulin of schizophrenia

目的 探讨精神分裂症患者血清半乳糖基化免疫球蛋白的定量变化及与血清总免疫球蛋白（Ig）及循环免疫复合物（CIC）含量的相关性.方法 收集2015年3月至2015年6月于解放军第261医院精神病科的住院患者共80例,排除中枢神经系统器质性病变与心、肝、肾等脏器疾病及其他自身免疫性疾病,其中男52例,女28例,年龄19~60岁,平均（27.2 ±6.2）岁.对照组选自同时期的80名健康体检者,其中男52名,女28名,年龄19~61岁,平均（27.8 ±5.4）岁.采用毡毛小脆柄菇凝集素（PVL）亲合ELISA法检测受试者血清中PVL亲合型Ig的数量变化.应用直线相关分析法研究患者组与对照组血清PVL-Ig与血清总Ig含量及CIC水平的相关性.结果 精神分裂症患者血清中PVL-IgG水平（A值）（3.128 ±0.531）明显高于对照组（A值）（2.672 ±0.492）（t=3.846,P〈0.01）;PVL-IgM（A值）（0.351 ±0.082）、PVL-IgA（A值）（1.287 ±0.205）较对照组（A值）（0.362 ± 0.073,1.203 ±0.216）,差异无统计学意义（t=0.241,0.6013;P〉0.05）;患者血清PVL-IgG水平与血清中CIC含量呈正相关（r=0.4231,P〈0.01）,其他指标（IgG、IgM、IgA）均无明显相关性.结论 精神分裂症患者有过量的PVL-IgG表达,可能参与了患者的免疫病理损伤过程;PVL-IgG可能是患者CIC滞留或清除障碍的分子机制之一.

Objective To investigate the correlation among serum galactose immunoglobulin of quantitative change, total IgG and CIC（circulating immune complexes）levels in patients with schizophrenia.Methods Test the number changed of PVL affinity type Ig in 80 patients with schizophrenia and 80 controls′serum with the PVL affinity ELISA method.The specimens without central nervous system organic disease,heart,liver,kidney diseases, and autoimmune diseases are collected from March to June 2015 in Hospital 261 of PLA′s hospitalized patients.The total of cases is 80.The cases of male are 52,and the cases of female are 28.The age is from 19 to 60,and the average of age is（27.2 ±6.2）.Control group is selected from health checkers from the same petiod.The cases of male are 52,and the cases of female are 28.The age is from 19 to 61,and the average of age is（27.8 ±5.4）.The relationships among serum PVL-Ig,total IgG and CIC levels in patient group and control group were investigated by rectilinear correlation analysis.Results The serum PVL-IgG level（3.128 ±0.531）（A value）in schizophrenia was significantly higher than control group（2.672 ±0.492）（A value）,（t=3.846,P〈0.01）.PVL-IgM（0.351 ±0.082）（A value）and PVL-IgA（1.287 ±0.205）（A value）compared with the control group（0.362 ±0.073,1.203 ± 0.216）（A value）, there was no difference（t=0.241,0.6013;P〉0.05）.There was positive correlation between serum PVL-IgG and CIC in patients（r=0.423 1,P〈0.01）.There was no significant correlation with IgG,IgM and IgA.Conclusions People with schizophrenia have an excess of PVL-IgG expression,which maybe participated in the pathological process of immunological injury.PVL-IgG may be one of the molecular mechanisms of patients′CIC stranded or cleared of obstacles.