谷胱甘肽S-转移酶基因在B型烟粉虱寄主转换中的差异比较

Comparison of the responses of glutathione S-transferase genes in Bemisia tabaci B to different host plant transfers

烟粉虱是一种世界性的重大农业害虫,其寄主范围十分广泛,对农作物危害严重。为研究GST基因家族在B型烟粉虱寄主转换中的响应机制,本文首先通过分析B型烟粉虱寄主转换后,23个GST基因表达量的变化,然后利用转录组数据、PCR、克隆、测序得到GST7基因全长,最后对GST7基因进行生物信息学分析。结果表明:烟粉虱GST7和GST13在辣椒寄主上的表达量与在原始寄主棉花上有显著差异,且GST7表达量上升了2.31倍;生物信息学分析结果显示GST7基因的开放阅读框全长657bp,编码219个氨基酸,没有信号肽,没有跨膜结构,理论等电点pI为4.81,其分子量为25.07kD,系统发育树分析表明GST7基因属于Delta亚家族。本研究发现GST7基因在烟粉虱适应不适寄主植物中起到一定作用,为将来RNAi研究GST7基因的功能奠定了基础。

The sweet potato whitefly Bemisia tabaci with wide host range is an important worldwide agricultural pest,causing serious damage to the crops.In order to study the response of glutathione S-transferases(GSTs)of B.tabaci B to host transfer,the expression of 23 GST genes were analyzed and then the transcriptome data,PCR and sequencing were used to get the ORF of GST7 gene.The bioinformation of GST7 was also elucidated.The results indicated that the expression levels GST7 and GST13 had significant difference in pepper compared with that in cotton(up to 2.31-fold for GST7).In addition,bioinformatics analysis showed that the open reading frame of GST7 gene was 657 bp,encoding 219 amino acids,and it contained no signal peptide and transmembrane structure.The theoretical isoelectric point(pI)was 4.81 and its molecular weight was 25.07 kD.The phylogenetic tree analysis showed that GST7 belonged to the Delta subfamily.These results suggest that GST7 gene may play an important role in host plant adaptation of B.tabaci B and provide a fundamental study for GST7 gene.