川佛手黄酮类物质的分离纯化和抗氧化活性研究

Study on separation,purification and antioxidant activity of flavonoids from chuan bergamot

采用微波和超声波联合提取技术提取川佛手中的黄酮.通过对大孔吸附树脂种类和洗脱液浓度条件优化,获得了最佳实验参数.在此基础上,选择D101树脂,通过石油醚、水和无水乙醇梯度洗脱对川佛手黄酮粗提物进行了分离纯化,得到川佛手黄酮纯化物纯度为92.65%.将川佛手黄酮粗产物、纯化产物、Vc和芦丁进行了抗氧化比较研究,川佛手黄酮纯化产物对DPPH·和·OH的清除率分别可达到82.22%和69.46%,结果表明川佛手黄酮纯化产物具有较好的清除自由基能力和较强的抗氧化活性.

Flavonoids were extracted from Chuan Bergamot by microwave and ultrasonic method.The optimum parameters were obtained by optimizing the macroporous resin and the concentration of eluent.On this basis,D101 was selected to separate and purify the crude flavonoids of Chuan Bergamot by gradient elution with petroleum ether,water and ethanol.The purity of the purified flavonoids was 92.65%.A comparative study was made on the antioxidant activity of Vc,rutin,crude flavonoids and purified flavonoids from Chuan Bergamot.The scavenging rates of DPPH · and ·OH were 82.22% and 69.46% from purified flavonoids of Chuan Bergamot.The results showed that the purified flavonoids from Chuan Bergamot had better scavenging ability and stronger antioxidant activity.