中国大陆地区2004-2016年柯萨奇病毒A组10型的分子流行病学特征分析

Molecular epidemiological analysis of coxsackievirus A10 strains isolated in China's Mainland from 2004 to 2016

目的分析中国大陆地区2004-2016年柯萨奇病毒A组10型(coxsackievirus group A type 10,CA10)流行毒株基因型分布概况和进化规律,为手足口病防控提供数据支撑。方法利用MEGA 6.0软件对GenBank核酸数据库收录的流行于中国大陆地区的CA10毒株VP1区基因序列进行分析,构建系统进化树,并对毒株彼此间的核苷酸和氨基酸同源性进行计算。结果纳入本研究的中国大陆地区CA10毒株共计218株,中国大陆地区CA10流行毒株的基因型以C型为主。与原型株CA10-Kowalik相比,中国大陆地区CA10毒株核苷酸和氨基酸的同源性分别为69.4%~73.9%和90.6%~93.6%;中国大陆地区CA10毒株内部的核苷酸和氨基酸同源性分别为76.4%~100.0%和92.9%~100.0%。结论本研究提示应针对CA10毒株流行趋势和基因型分布情况,采取有效措施防控手足口病。

Objective To analyze the genotype distribution and genetic evolution of coxsackievirus A10（ CA10）isolated in China's Mainland,2004-2016,and to provide a scientific basis supporting for prevention and control of handfoot-and-mouth disease. Method MEGA 6. 0 software was used to analyze the VP1 gene sequence of CA10 isolates from China's Mainland through retrieving the Gen Bank nucleotide database. Besides,the phylogenetic tree was constructed and the homology of nucleotide and amino acids were calculated. Results A total of 218 Chinese CA10 isolates were included in this study. Most of Chinese CA10 isolates emerged from 2004 to 2016 belonged to genotype C. Comparing with the ptototype strain CA10-Kowalik,the homology of nucleotide sequences and amino acid sequences of VP1 gene of 218 Chinese isolates were 69. 4%-73. 9% and 90. 6%-93. 6%,respectively; Moreover,the nucleotide and the amino acid homology of VP1 gene of 218 Chinese CA10 isolates among themselves ranged from 76. 4% to 100. 0% and ranged from 92. 9% to100. 0%,respectively. Conclusions This study suggests that effective measures should be taken to prevent hand-footand-mouth disease according to the tendency of molecular epidemiology and genotype distribution of CA10 strains isolated in China.