病理性瘢痕成纤维细胞中凋亡因子Fas、Bcl-2、survivin、caspase-3蛋白的表达及意义

The Expression and Significance of Apoptotic Factors Fas,Bcl-2,Survivin and Caspase-3 Protein in Pathological Scar Fibroblasts

目的:探讨病理性瘢痕成纤维细胞中凋亡因子Fas、Bcl-2、survivin、caspase-3蛋白的表达及意义。方法:采用Western bolt技术检测46例病理性瘢痕组织、25例非病理性瘢痕组织和22例正常皮肤组织中凋亡相关因子Fas、Bcl-2、survivin、caspase-3的阳性表达率,并分析其相关性。结果:病理性瘢痕成纤维组织中Fas、caspase-3阳性表达率分别为21.74%、23.91%低于非病理性瘢痕的60.0%、72.0%和正常皮肤组织的86.36%、90.90%,差异有统计学意义(P<0.05);病理性瘢痕成纤维组织Bcl-2、survivin阳性表达率分别为78.26%、82.61%高于非病理性瘢痕的44.0%、48.0%和正常皮肤组织的13.64%、18.18%,差异有统计学意义(P<0.05);且非病理性瘢痕和正常皮肤组织中上述指标也存在明显差异(P<0.05);Spearman相关分析显示,Fas蛋白与Bcl-2蛋白、survivin蛋白表达呈负相关关系(r=-0.513、-0.629,P<0.05),与caspase-3蛋白表达呈正相关关系(r=0.691,P<0.05);Bcl-2蛋白表达与survivin蛋白表达呈正相关关系(r=0.6.16,P<0.05),与caspase-3蛋白表达呈负相关关系(r=-0.638,P<0.05),survivin蛋白表达与caspase-3蛋白表达呈负相关关系(r=-0.621,P<0.05)。结论:病理性瘢痕成纤维组织中凋亡相关因子Fas、Bcl-2、survivin、caspase-3的异常表达,可能参与病理性瘢痕的形成。

Objective To investigate the expression and significance of apoptotic factors Fas, Bcl-2, survivin and caspase-3 protein in pathological scar fbroblasts. Methods The positive expression rates of apoptosis-associated factors Fas, Bcl-2, survivin and Caspase-3 protein in 46 cases of pathological scar tissues, 25 cases of non-pathological scar tissues and 22 cases of normal skin tissues were detected by Western bolt, and the correlation was analyzed. Results The positive expression rates of Fas and caspase-3 in pathological scar fbroblasts （21.74%, 23.91%） were lower than those in the non-pathological scar （60.0%, 72.0%） or those in normal skin tissues （86.36%, 90.90%） （P〈0.05）, while the positive expression rates of Bcl-2 and survivin in pathological scar fbroblasts （78.26%, 82.61%） were signifcantly higher than those in non-pathological scars （44.0%, 48.0%） or those in normal skin tissues （13.64%, 18.18%） （P〈0.05）. There were signifcant differences in above-mentioned indexes between non-pathological scars and normal skin tissues （P〈0.05）. Spearman correlation analysis showed that Fsa protein was negatively related to Bcl-2 protein and survivin protein （r=-0.513, -0.629, P〈0.05）, and positively correlated with caspase-3 protein （r=0.691, P〈0.05）. Bcl-2 protein was positively correlated survivin protein （r=0.6.16, P〈0.05）, and negatively correlated with caspase-3 protein （r=-0.638, P〈0.05）. The survivin protein was negatively correlated with caspase-3 protein （r=-0.621, P〈0.05）. Conclusion The abnormal expression of apoptosis related factors Fas, Bcl-2, survivin and caspase-3 in pathological scar fbroblasts may be involved in the occurrence of pathological scar.