正常与性早熟中华绒螯蟹的Y-器官转录组分析

Transcriptome sequencing and functional analysis of Y-organs between normal and precocious Chinese mitten crabs(Eriocheir sinensis)

性早熟是中华绒螯蟹养殖过程中普遍存在的一个问题。为发掘中华绒螯蟹性早熟相关的重要功能基因,实验采用Illumina Hiseq 2000高通量测序技术获得了正常与性早熟雌蟹的Y-器官转录组数据,并进行比较分析。结果显示,测序分别获得44 619 538和43 052 958个clean reads,比对发现2655个差异表达基因,Gene Ontology(GO)功能分类分析将文库中的差异表达基因归类到3大功能(生物过程、细胞组分和分子功能)的42个类别中。KEGG富集分析将文库中的差异表达基因富集到134条特定的KEGG代谢途径,其中4条是显著性富集,包括酮体合成和降解、丁酸甲酯代谢、神经营养因子信号通路和碱基切除修复通路。通过RNA-seq技术获得了丰富的中华绒螯蟹Y-器官转录组信息,为中华绒螯蟹新基因克隆、性早熟成因与预防和Y-器官的研究提供有价值的数据。

Precocity is a common problem in aquaculture of Chinese mitten crab（Eriocheir sinensis）. To discover and clone important functional genes, the transcriptome of Y-organs of precocious and normal female crabs were sequenced by Illumina Hiseq 2000 high-throughput sequencing technology, and comparative transcriptome study was conducted. The results showed that precocious and normal crabs had 44 619 538 and 43 052 958 clean reads,respectively. Comparison between the sequencing data from precocious and normal female crabs revealed 2 655 differentially expressed genes. The differentially expressed genes GO functions in the transcriptome library were broadly divided into three major ontology（biological processes, cellular component and molecular function categories） of 42 branches. Data in the transcriptome from 134 differentially expressed genes could be divided into4 classes taking the KEGG database as a reference, according to the metabolic pathway, including synthesis and degradation of ketone bodies, butanoate metabolism, neurotrophin signaling pathway, and base excision repair. By RNA-seq, we obtained abundant transcriptome information that could contribute to novel gene identification,causes and prevention of precocity, and research of Y-organs in Eriocheir sinensis.