血液增菌肉汤连续传代对多药耐药菌恢复抗菌药物敏感性研究

Recovery of sensitivity of multidrug-resistant bacteria to antibacterial drugs by continuous regeneration of blood-enhanced broth

目的探讨连续传代培养对产ESBLs多药耐药菌恢复药物敏感性的影响。方法选择临床产ESBLs的大肠杆菌和肺炎克雷伯杆菌各21株,以血液增菌肉汤连续30代接种传代。对连续传代且无杂菌污染的菌株进行体外药敏试验。结果共获得纯化的产ESBLs的大肠杆菌和肺炎克雷伯杆菌分别为15株和18株,传30代结果显示共有21株51种次的抗菌药物由耐药变成敏感,其敏感性恢复了,4株6种次由敏感变成耐药。结论血液增菌肉汤连续30代接种传代可诱导部分产ESBLs肺炎克雷伯菌、大肠埃希菌恢复药物敏感性。

OBJECTIVE To investigate the effect of continuous subculture on the recovery of drug sensitivity of ESBLs-producing multidrug-resistant bacteria.METHODS A total of 21 strains of ESBLs-producing Escherichia coli and 21 strains of ESBLs-producing Klebsiella pneumoniae were selected,and the blood enhanced broth was inoculated for 30 generations.The in vitro drug susceptibility test was carried out for strains which were continuously passaged and had no contamination.RESULTS A total of 15 strains of purified ESBLs-producing E.coli and 18 strains of purified ESBLs-producing K.pneumoniae were obtained.The results showed that,after 30 generations of subculture,21 strains of 51 species were sensitive to antibacterial drugs and their sensitivity was restored,and4 strains of 6 species turned from sensitivity to resistance.CONCLUSIONBlood-enhanced broth can induce partial ESBLs-producing K.pneumoniae and E.coli to restore drug sensitivity after 30 generations of subculture.