气体ClO_2对猕猴桃青霉的抑菌作用及其机理

Bacteriostasis and antimicrobial mechanism of chlorine dioxide gas on penicillium sp. of Kiwi fruit

以生长处于对数期的猕猴桃青霉为实验对象,研究气体ClO_2对猕猴桃青霉的抑制作用及主要细胞内容物渗漏的影响。结果表明:气体ClO_2对青霉孢子的萌发与菌丝体的生长有较好的抑制作用,0.8mg/L处理7min及0.6mg/L处理10min均可完全抑制猕猴桃青霉孢子的萌发;2.8mg/L处理25min可以完全抑制青霉菌丝体的生长。气体ClO_2损害了青霉细胞屏障功能及细胞膜的渗透性,气体ClO_2对猕猴桃青霉蛋白质、DNA、K^+的渗漏量影响较大,与青霉的抑制率相关性较强,可能是抑制青霉的重要原因;而气体ClO_2对猕猴桃青霉Mg^(2+)、Ca^(2+)的渗透量影响较小。

Taking Penicillium sp.of Kiwi Fruit as experimental subjects,the bacteriostasis of chlorine dioxide gas on both Penicilliumsp.and the leakage of main cellular content of kiwi fruit are studied.The results shows that chlorine dioxide gas could both inhibit the germination of Penicilliumsp.spores and the growth of mycelium.The germination of Penicilliumsp.spores were completely inhibited when the kiwi fruit was treated with chlorine dioxide gas about 0.8 mg/L for 7 minutes and about 0.6 mg/L for 10 minutes.And a 25-minute treatment of chlorine dioxide gas under the concentration of 2.8 mg/L might exterminate the growth of mycelium.Chlorine dioxide gas damaged the barrier function and the membrane permeability of Penicillium sp.cells.No matter how long the time of treatment of Penicilliumsp.toward kiwi fruit would be,there is always a strong correlation between the leakage of protein,DNA and the inhibition rate of Penicilliumsp.,which may be the important reason of inhibiting Penicilliumsp.Meanwhile,there are minor effects of chlorine dioxide gas on the leakage of magnesium ions and calcium ions.